2024
Biochemical and structural characterization of a class A β-lactamase from Nocardia cyriacigeorgica. Feuillard J, Couston J, Benito Y, Hodille E, Dumitrescu O, Blaise M. Acta Crystallogr F Struct Biol Commun. 2024 Jan 1;80(Pt 1):13-21. doi: 10.1107/S2053230X23010671
2023
Cryo-EM structure of the trehalose monomycolate transporter, MmpL3, reconstituted into peptidiscs. Couston J, Guo Z, Wang K, Gourdon P, Blaise M. Curr Res Struct Biol. 2023 Nov 8;6:100109. doi: 10.1016/j.crstbi.2023.100109.
Structural basis of the mycobacterial stress-response RNA polymerase auto-inhibition via oligomerization.
Morichaud Z, Trapani S, Vishwakarma RK, Chaloin L, Lionne C, Lai-Kee-Him J, Bron P, Brodolin K. Nat Commun. 2023 Jan 30;14(1):484. doi: 10.1038/s41467-023-36113-y
. PMID: 36717560 Free PMC article.
A Tripartite Complex HIV-1 Tat-Cyclophilin A-Capsid Protein Enables Tat Encapsidation That Is Required for HIV-1 Infectivity.
Schatz M, Marty L, Ounadjela C, Tong PBV, Cardace I, Mettling C, Milhiet PE, Costa L, Godefroy C, Pugnière M, Guichou JF, Mesnard JM, Blaise M, Beaumelle B. J Virol. 2023 Apr 27;97(4):e0027823. doi: 10.1128/jvi.00278-23. Epub 2023 Apr 11. PMID: 37129415
Second-Generation CD73 Inhibitors Based on a 4,6-Biaryl-2-thiopyridine Scaffold.
Ghoteimi R, Braka A, Rodriguez C, Cros-Perrial E, Duvauchelle V, Uttaro JP, Mathé C, Ménétrier-Caux C, Jordheim LP, Chaloin L, Peyrottes S. ChemMedChem. 2023 Apr 3;18(7):e202200594. doi: 10.1002/cmdc.202200594. Epub 2023 Feb 7. PMID:
36700491
2022
An evolutionarily conserved N-terminal leucine is essential for MX1 GTPase antiviral activity against different families of RNA viruses.
McKellar J, Arnaud-Arnould M, Chaloin L, Tauziet M, Arpin-André C, Pourcelot O, Blaise M, Moncorgé O, Goujon C.J Biol Chem. 2023 Jan;299(1):102747. doi: 10.1016/j.jbc.2022.102747. Epub 2022 Nov 25.PMID: 36436557 Free PMC article.
Identification of a non-canonical G3BP-binding sequence in a Mayaro virus nsP3 hypervariable domain.
Neyret A, Bernard E, Aïqui-Reboul-Paviet O, Bakhache W, Eldin P, Chaloin L, Briant L. Front Cell Infect Microbiol. 2022 Aug 11;12:958176. doi: 10.3389/fcimb.2022.958176. eCollection 2022. PMID: 36034716 Free PMC article.
Singular Interface Dynamics of the SARS-CoV-2 Delta Variant Explained with Contact Perturbation Analysis.
Gheeraert A, Vuillon L, Chaloin L, Moncorgé O, Very T, Perez S, Leroux V, Chauvot de Beauchêne I, Mias-Lucquin D, Devignes MD, Rivalta I, Maigret B. J Chem Inf Model. 2022 Jun 27;62(12):3107-3122. doi: 10.1021/acs.jcim.2c00350. Epub 2022 Jun 6. PMID: 35754360 Free PMC article.
Biochemical, structural, and functional studies reveal that MAB_4324c from Mycobacterium abscessus is an active tandem repeat N-acetyltransferase.
Alsarraf HMAB, Ung KL, Johansen MD, Dimon J, Olieric V, Kremer L, Blaise M. FEBS Lett. 2022 Jun;596(12):1516-1532. doi: 10.1002/1873-3468.14360. Epub 2022 Jun 3. PMID: 35470425
The DEAD box RNA helicase DDX42 is an intrinsic inhibitor of positive-strand RNA viruses.
Bonaventure B, Rebendenne A, Chaves Valadão AL, Arnaud-Arnould M, Gracias S, Garcia de Gracia F, McKellar J, Labaronne E, Tauziet M, Vivet-Boudou V, Bernard E, Briant L, Gros N, Djilli W, Courgnaud V, Parrinello H, Rialle S, Blaise M, Lacroix L, Lavigne M, Paillart JC, Ricci EP, Schulz R, Jouvenet N, Moncorgé O, Goujon C. EMBO Rep. 2022 Nov 7;23(11):e54061. doi: 10.15252/embr.202154061. Epub 2022 Sep 26. PMID: 36161446Free PMC article.
MmpL3, the trehalose monomycolate transporter, is stable in solution in several detergents and can be reconstituted into peptidiscs.
Ung KL, Alsarraf H, Kremer L, Blaise M. Protein Expr Purif. 2022 Mar;191:106014. doi: 10.1016/j.pep.2021.106014. Epub 2021 Nov 9. PMID: 34767949
Crystal structure of the TLDc domain of human NCOA7-AS.
Arnaud-Arnould M, Tauziet M, Moncorgé O, Goujon C, Blaise M. Acta Crystallogr F Struct Biol Commun. 2021 Aug 1;77(Pt 8):230-237. doi: 10.1107/S2053230X21006853. Epub 2021 Jul 28. PMID: 34341188 Free PMC article.
Cytotoxic and Antitumoral Activity of N-(9H-purin-6-yl) Benzamide Derivatives and Related Water-soluble Prodrugs.
Cros-Perrial E, Saulnier S, Raza MZ, Charmelot R, Egron D, Dumontet C, Chaloin L, Peyrottes S, Jordheim LP. Curr Mol Pharmacol. 2022 ;15(6):883-894. doi: 10.2174/1874467214666211014164406. PMID: 34649495
2021
Region 4 of the RNA polymerase σ subunit counteracts pausing during initial transcription.
Brodolin K, Morichaud Z. J Biol Chem. 2021 Jan-Jun;296:100253. doi: 10.1074/jbc.RA120.016299. Epub 2021 Jan 8. PMID: 33380428Free PMC article.
Modular Imaging Scaffold for Single-Particle Electron Microscopy.
Aissaoui N, Lai-Kee-Him J, Mills A, Declerck N, Morichaud Z, Brodolin K, Baconnais S, Le Cam E, Charbonnier JB, Sounier R, Granier S, Ropars V, Bron P, Bellot G. ACS Nano. 2021 Mar 23;15(3):4186-4196. doi: 10.1021/acsnano.0c05113. Epub 2021 Feb 15. PMID: 33586425
4-Substituted-1,2,3-triazolo nucleotide analogues as CD73 inhibitors, their synthesis, in vitro screening, kinetic and in silico studies.
Ghoteimi R, Braka A, Rodriguez C, Cros-Perrial E, Tai Nguyen V, Uttaro JP, Mathé C, Chaloin L, Ménétrier-Caux C, Jordheim LP, Peyrottes S. Bioorg Chem. 2021 Feb;107:104577. doi: 10.1016/j.bioorg.2020.104577. Epub 2020 Dec 23. PMID: 33450542
2020
The σ Subunit-Remodeling Factors: An Emerging Paradigms of Transcription Regulation.
Vishwakarma RK, Brodolin K. Front Microbiol. 2020 Jul 29;11:1798. doi: 10.3389/fmicb.2020.01798. eCollection 2020. PMID: 32849409 Free PMC article. Review.
Dihydropyrimidinase protects from DNA replication stress caused by cytotoxic metabolites.
Basbous J, Aze A, Chaloin L, Lebdy R, Hodroj D, Ribeyre C, Larroque M, Shepard C, Kim B, Pruvost A, Moreaux J, Maiorano D, Mechali M, Constantinou A. Nucleic Acids Res. 2020 Feb 28;48(4):1886-1904. doi: 10.1093/nar/gkz1162. PMID: 31853544 Free PMC article.
Mitigation of heterocyclic aromatic amines in cooked meat. Part I: Informed selection of antioxidants based on molecular modeling.
Meurillon M, Angénieux M, Mercier F, Blinet P, Chaloin L, Chevolleau S, Debrauwer L, Engel E. Food Chem. 2020 Nov 30;331:127264. doi: 10.1016/j.foodchem.2020.127264. Epub 2020 Jun 7. PMID: 32619906Free article.
Extracellular NAD+ enhances PARP-dependent DNA repair capacity independently of CD73 activity.
Wilk A, Hayat F, Cunningham R, Li J, Garavaglia S, Zamani L, Ferraris DM, Sykora P, Andrews J, Clark J, Davis A, Chaloin L, Rizzi M, Migaud M, Sobol RW. Sci Rep. 2020 Jan 20;10(1):651. doi: 10.1038/s41598-020-57506-9.PMID: 31959836Free PMC article.
2019
Guillon R., Rahimova R., Preeti, Egron D., Rouanet S., Dumontet C., Aghajari N., Jordheim L.P., Chaloin L. and Peyrottes S., “Lead optimization and biological evaluation of fragment-based cN-II inhibitors” J. Med. Chem. (2019), 168, 28-44 co-corresponding authors
Ghoteimi R., Nguyen V.T., Rahimova R., Grosjean F., Cros-Perrial E., Uttaro J-P., Mathé C., Chaloin L., Jordheim L.P., Peyrottes S. “Synthesis of Substituted 5′-Aminoadenosine Derivatives and Evaluation of Their Inhibitory Potential toward CD73.” ChemMedChem. (2019), 14, 1431-1443. doi: 10.1002/cmdc.201900348.
Fernandez J., Machado A., Lyonnais S., Chamontin C., Gärtner K., Léger T., Henriquet C., Garcia C., Portilho D.M., Pugnière M., Chaloin L., Muriaux D., Yamauchi Y., Blaise M., Nisole S., Arhel N.J. “Transportin-1 binds to the HIV-1 capsid via a nuclear localization signal and triggers uncoating” Nature Microbiology (2019), 10.1038/s41564-019-0575-6
2018
Sudalaiyadum Perumal A, Vishwakarma RK, Hu Y, Morichaud Z, Brodolin K. “RbpA relaxes promoter selectivity of M. tuberculosis RNA polymerase.” Nucleic Acids Res. (2018) 46(19):10106-10118. doi: 10.1093/nar/gky714. 
Vishwakarma RK, Cao AM, Morichaud Z, Perumal AS, Margeat E, Brodolin K. “Single-molecule analysis reveals the mechanism of transcription activation in M. tuberculosis.” Sci Adv. (2018) 4 (5):eaao5498. doi: 10.1126/sciadv.aao5498. PubMed
Rahimova R., Fontanel S., Lionne C., Jordheim L.P., Peyrottes S. and Chaloin L. “Identification of allosteric inhibitors of the ecto-5’-nucleotidase (CD73) targeting the dimer interface”. PLoS Comp. Biol. (2018), doi 10.1371/journal.pcbi.1005943
Dulin D, Bauer DLV, Malinen AM, Bakermans JJW, Kaller M, Morichaud Z, Petushkov I, Depken M, Brodolin K, Kulbachinskiy A, Kapanidis AN. “Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria.” Nat Commun. (2018) 9 (1):1478. doi: 10.1038/s41467-018-03902-9. PubMed
2017
Duchi D, Gryte K, Robb NC, Morichaud Z, Sheppard C, Brodolin K, Wigneshweraraj S, Kapanidis AN. “Conformational heterogeneity and bubble dynamics in single bacterial transcription initiation complexes.” Nucleic Acids Res. (2017) Nov 21. doi: 10.1093/nar/gkx1146. Pubmed
Leban, N., Kaplan E., Chaloin L., Godreuil S. and Lionne C. “Kinetic characterization and molecular docking of novel allosteric inhibitors of aminoglycoside phosphotransferases” Biochim Biophys Acta. (2017), 1861, 3464-3473. Pubmed
Gissot M., Hovasse A., Chaloin L., Schaeffer-Reiss C., Van Dorsselaer A. and Tomavo S. “An evolutionary conserved zinc finger protein is involved in Toxoplasma gondii mRNA nuclear export.” Cell. Microbiol. (2017) doi: 10.1111/cmi.12644. Pubmed
Baud A, Aymé L, Gonnet F, Salard I, Gohon Y, Jolivet P, Brodolin K, Da Silva P, Giuliani A, Sclavi B, Chardot T, Mercère P, Roblin P, Daniel R. “SOLEIL shining on the solution-state structure of biomacromolecules by synchrotron X-ray footprinting at the Metrology beamline.” J Synchrotron Radiat. (2017) 24(Pt 3):576-585. doi: 10.1107/S1600577517002478.
2016
Morichaud Z., Chaloin L. and Brodolin K. “Regions 1.2 and 3.2 of the RNA polymerase subunit promote DNA melting and attenuate action of the antibiotic lipiarmycin.” J. Mol. Biol. (2016) 428, 463-76. Pubmed
Duchi D, Bauer DL, Fernandez L, Evans G, Robb N, Hwang LC, Gryte K, Tomescu A, Zawadzki P, Morichaud Z, Brodolin K, Kapanidis AN. “RNA Polymerase Pausing during Initial Transcription”. Mol Cell. (2016) – 63(6):939-50. Pubmed
Nasri A., Valverde A., Roche D.B., Desrumaux C., Clair P., Beyrem H., Chaloin L., Ghysen A. and Perrier V. “Neurotoxicity of a Biopesticide Analog on Zebrafish Larvae at Nanomolar Concentrations” Int. J. of Mol. Sci., (2016), 17 (12). Pubmed
Kaplan E., Guichou J.F., Chaloin L., Kunzelmann S., Leban N., Serpersu E.H. and Lionne C. “Aminoglycoside binding and catalysis specificity of aminoglycoside 2”-phosphotransferase IVa: a thermodynamic, structural and kinetic study” Biochim Biophys Acta. (2016) 1860, 802-813. Pubmed
Nguyen V.T., Hospital, A., Lionne C., Jordheim L.P., Dumontet C., Périgaud C., Chaloin L. and Peyrottes S. “Beta-hydroxyphosphonate ribonucleoside analogues derived from 4-substituted-1,2,3-triazoles as IMP/GMP mimics: synthesis and biological evaluation » Beilstein J. Org. (2016), 12, 1476-1486. Pubmed
2015
Marton Z., Guillon R., Krimm I., Preeti, Rahimova R., Egron D., Jordheim L.P., Aghajari N., Dumontet C., Périgaud C., Lionne C., Peyrottes S. and Chaloin L. “Identification of non-competitive inhibitors of cytosolic 5′-nucleotidase II using a fragment-based approach.” J. Med Chem. (2015) 58, 9680-96. Pubmed
Borel S., Robert-Hebmann V., Alfaisal J., Jain A., Faure M., Espert L., Chaloin L., Paillart J.-C., Johansen T. and Biard-Piechaczyk M. “HIV-1 Vif interacts with LC3 and inhibits autophagy” AIDS (2015) 29, 275-286. Pubmed
Cividini F., Pesi R., Chaloin L., Allegrini S., Camici M., Cros-Perrial E., Dumontet C., Jordheim L.P., Tozzi M.G. “The purine analog fludarabine acts as a cytosolic 5′-nucleotidase II inhibitor” Biochem Pharmacol. (2015) 94, 63-68. Pubmed
Briolotti P., Chaloin L., Balaguer P., Da Silva F., Tománková V., Pascussi J.-M., Duret C., Fabre J.-M., Ramos J., Klieber S., Maurel P., Daujat-Chavanieu M., Gerbal-Chaloin S. “Analysis of glycogen synthase kinase inhibitors that regulate cytochrome P450 expression in primary human hepatocytes by activation of β-catenin, aryl hydrocarbon receptor and pregnane X receptor signaling” Toxicol Sci. (2015) 148, 261-75. Pubmed
Ramya L., Gautham N., Chaloin L., Kajava A.V. “Restricted mobility of side chains on concave surfaces of solenoid proteins may impart heightened potential for intermolecular interactions.” Proteins (2015) 83, 1654-64. Pubmed
Brevets
