2 Postdoctoral positions on innate immunity and respiratory viruses to work on the ERC-funded project InVIRium led by Caroline Goujon
The Interferon and Antiviral Restriction Lab, led by Caroline Goujon, is recruiting 2 postdoctoral fellows on innate immunity and respiratory viruses to work on the ERC-funded InVIRium project, for up to 4 and 5 years, respectively:
– 1 postdoctoral fellow with a strong expertise in the fields of innate immunity and respiratory viruses (experience in BSL-3 lab, and with respiratory viruses required). Experience in 3D-cultures of primary airway epithelia would be a massive plus.
– 1 postdoctoral fellow with skills in bioinformatics (scRNA-seq and NGS analyses) and in wet lab experiments, with expertise in molecular virology.
The InVIRium project aims at understanding the relationships between respiratory viruses and their host cells, using highly pertinent models of airway epithelia cultured at the air-liquid interface, with a major focus on respiratory viruses (coronaviruses, influenza viruses and respiratory syncytial virus) and on interferon-induced antiviral restriction.
The 2 positions will be available from the beginning of 2024. Candidates should send their application, CV and contact information for at least 2 referees to Caroline Goujon.
Post-doctoral position (2 years) in Nuclear pore biology & Immunity in Montpellier, France
The research team “Viral Trafficking, Restriction and Innate Signaling” (VTRIS) studies virus-host cell interactions, with a focus on antiviral innate immunity and nuclear pore biology. Our models are human RNA viruses, including HIV-1, Influenza virus and flaviviruses.
We have an ANR-funded post-doctoral position to investigate the role of the nuclear pore component RanBP2 in inflammatory responses to viral infection. Mutations in the N-terminal domain of RanBP2 are associated with a rare genetic predisposition to deleterious inflammation and Acute Necrotizing Encephalopathy (ANE) following viral infection, frequently by Influenza A virus (IAV). This project seeks to uncover the mechanisms linking RanBP2 to deleterious inflammation following IAV infection by examining the CNS-specific role of RanBP2 and brain susceptibility of ANE. This project will help understand how alterations in the nuclear pore complex contribute to disease development following viral infection.
For this project, we are seeking a full-time post-doctoral researcher who will explore innate immune mechanisms in CRISPR-edited human cells and mouse models. The main techniques will include immunohistochemistry, flow cytometry/Legendplex, multiplex quantitative PCR and RNAscope in situ hybridization. Candidates must have a PhD in biological or biomedical sciences preferably in virology, immunology, or neurology. The delivery date for the PhD diploma should be between 2017 and 2024. Strong competence in molecular biology and an interest in neurogenetics would be an asset.
The candidate will integrate the IRIM institute, a lively and productive research environment entirely dedicated to research on infectious diseases. Estimated start date: January 2024. Estimated gross salary: 2,934 – 4,122 €/month depending on previous working experience.
Applicants are invited to send a cover letter and CV with the name of referees to Dr. Nathalie Arhel ().
POST-DOC POSITION IN MOUGEL’s TEAM (R2D2) IN VIRUS-CELL INTERACTION AND REAL-TIME MICROSCOPY
We are looking for an outstanding, highly-motivated candidate to join our team to develop a new project entitled: « Real time imaging of single viral RNA translation: understanding further how murine leukemia viruses (MLV) hijack the cellular pathways! ».
Understanding how retroviruses manipulate the cellular functions to replicate is crucial to design anti-viral therapies. Research project will aim to set up advanced technologies of real-time fluorescence microscopy (SunTag, SunRISE) at the scale of single molecule of viral RNA to understand MLV expression in cells. The project will be realized with the collaboration of Philippe Pierre’s lab in Marseille who have developed these techniques.
Candidates bringing expertise in fluorescence microscopy and image analysis are particularly welcome to apply. Prior research experience on virology would be an asset, but is not essential.
Starting date end 2019 (very flexible); 2-year contract financed by Infectiopole Sud.
Visit Infectiopole website for more information on eligibility criteria: www.infectiopolesud.com/bourses.html
Please send your full application including motivation letter, CV and university marks (notes and ranking), list of publications and the names and email addresses of 2-3 referees to: marylene.mougel[at]irim.cnrs.fr as a single PDF file with “Postdoc application” in the subject.
Post-doc position in cell biology – EV biology – membrane trafficking
We are looking for a motivated post-doctoral scientist to join the research team “Membrane dynamics & viruses” (MDV) headed by Raphael Gaudin, part of the IRIM UMR9004 – CNRS research institute, Montpellier, France. Our lab is interested in various aspects of intracellular trafficking pathways in health and disease. In particular, we are currently studying secretion of biological entities out of the cells, including extracellular vesicles and viruses. We recently discovered a novel extracellular vesicle subtype containing Sonic Hedgehog (Shh) we called ART-EVs (Coulter, Dorobantu et al. Cell Rep, 2018) and we aim to better characterize these vesicles and their secretory route. The proposed project will take advantage of state-of-the-art imaging techniques, Crispr-Cas9 strategies and other innovative approaches mastered in the lab.
The successful candidate is expected to perform bench work and communicate his/her results through internal and external seminars. The project will be conducted in collaboration with two other labs with in vivo and proteomics expertise.
You are going to defend or already hold a PhD in Cell biology with experience in membrane trafficking. Previous experience with exosomes or extracellular vesicles is a plus. You are curious, motivated and have a problem-solving mindset. The applicant is expected to speak English, while French is not a prerequisite.
The contract is for 1-year renewable. Starting date early-mid 2019. Applications must be sent to R. Gaudin: raphael.gaudin(at)irim.cnrs.fr
3-year postdoc position available on innate immunity and viruses Interferon and Antiviral Restriction Lab (C. Goujon’s team)*
Institut de Recherche en Infectiologie de Montpellier, France
The Interferon and Antiviral Restriction Lab, led by Caroline Goujon, is recruiting a talented and highly motivated postdoctoral fellow with expertise in the fields of innate immunity and/or virology to work on the ANTIViR ERC-funded project. This project aims at understanding the mechanisms of interferon-induced antiviral restriction and signaling, focusing on two major pathogenic viruses, HIV-1 and influenza A virus.
Applicants must hold a PhD degree in Life sciences. An excellent knowledge of influenza A virus biology and/or innate immunity is absolutely required, and expertise in super resolution microscopy and/or bioinformatics would be a plus.
Candidates should send their application, CV (including a publication list) and contact of 2 to 3 referees to:
Deadline: December 2018
Starting: January / February 2018
Tomas Doyle, Olivier Moncorgé, Boris Bonaventure, Darja Pollpeter, Marion Lussignol, Marine Tauziet, Luis Apolonia, Maria-Teresa Catanese, Caroline Goujon* and Michael H. Malim* (* co-senior, co-corresponding authors). The interferon inducible isoform of NCOA7 inhibits endosome-mediated viral entry. Nature Microbiology, in press (http://dx.doi.org/10.1038/s41564-018-0273-9).
Doyle, T., Goujon, C., and Malim, M.H. (2015). HIV-1 and interferons: who’s interfering with whom? Nature Reviews Microbiology 13, 403-413.
Post-Doctoral position in phosphate homeostasis and calcification at IRIM, Montpellier, France
A post-doctoral position funded by the French National Agency of research (ANR) is open for a talented and highly motivated post-doc fellow to study cellular phosphate homeostasis and its misregulation leading to vascular calcification.
Phosphate is an important mineral for the synthesis of membranes and nucleic acids, for energy production and signal transduction, and its concentration is tightly regulated by phosphate transporters. Little is known about how human and other metazoan cells sense phosphate to regulate phosphate homeostasis and metabolism. We are particularly interested in XPR1, a retroviral receptor that we found to mediate phosphate efflux and to be associated with a rare disease called primary familial brain calcification (PFBC). The objective of the post-doctoral project is to understand how XPR1 is integrated in this regulated sensing process and why mutations in the XPR1 gene can lead to phosphate-associated disorders like calcification. The successfull applicant will use a broad range of technics, in particular genome modifications and genetic screens, solute transport assays, as well as calcification assays in in vitro and in vivo models..
1- Giovannini D., Touhami J., Charnet P., Sitbon M.*, Battini JL.* 2013. Inorganic phosphate export by the retrovirus receptor XPR1 in metazoans. 2013. Cell Reports 3: 1866-73.
2- Legati A., Giovannini D., …, Geschwind DH., Battini JL.*, Coppola G*. 2015. Mutations in XPR1 cause primary familial brain calcification associated with altered phosphate export. Nature Genetics 47: 579–581.
The candidate will be part of the CALCIPHOS ANR project, organized as an interdisciplinary research program between the Labs of Jean-Luc Battini/Laurence Briant (IRIM, CNRS UMR9004, Montpellier), Marc Sitbon (IGMM, CNRS UMR5535, Montpellier) and Gaël Nicolas/Dominique Campion (INSERM U1245, Rouen). She/he will benefit from a highly dynamic and collaborative environment at the « route de Mende » CNRS campus of Montpellier and from state-of-the-art technological platforms.
Candidates are expected to be autonomous, enthusiastic, and able to work in a collaborative team. Experience in cell and molecular biology, biochemistry and imaging is required as well as good communication skills in english. An expertise in animal studies and histology as well as a background in calcification/mineralization would be appreciated. The position is funded for 2 years and salary will be based on experience.
Applicants must hold a PhD degree in biology and should send a letter of scientific achivements and of their interest in this project, a CV with list of publications, and contact informations of 2 referees to jean-luc.battini(a)irim.cnrs.fr
Deadline: December 2017
Starting: January / February 2018
L’équipe de Laurent Kremer recherche un post-doc en mycobactériologie pour 2 ans dans le cadre d’un projet financé par le LabEx EpiGenMed.
La paroi mycobactérienne représente une structure complexe constituée de lipides atypiques qui agissent comme immunomodulateurs et facteurs de virulence. Une meilleure compréhension des enzymes impliquées dans l’export de ces lipides nous renseignerait sur la biogenèse de la paroi.
Nous avons récemment obtenu des informations fonctionnelles et structurales sur une famille de transporteurs lipidiques, les protéines de la famille MmpL, qui appartiennent à la superfamille des pompes à efflux. Mycobacterium abscessus, un pathogène émergent dans la mucoviscidose possède au moins 29 MmpLs dont les fonctions restent largement inexplorées. Ce projet consistera à générer des mutants dans des gènes codant des MmpLs et d’analyser leur phénotype en relation avec (i) la biosynthèse de la paroi ; (ii) la contribution individuelle de chacun de ces transporteurs dans la phagocytose et la survie intramacrophagique; (iii) leur rôle dans physiopathologie des infections à M. abscessus dans l’embryon de zebrafish; (iv) leur implication dans la résistance/susceptibilité aux antibiotiques.