Fonctions
Directeur de Recherche 2- CNRS
HDR
Equipe
Enzymes bactériennes et résistance aux antibiotiques
Contact
email : mickael.blaise(at)irim.cnrs.fr
Tél : 04 34 35 94 68
ORCID: https://orcid.org/0000-0002-7860-3464
Publications (5 dernières années )
2024
Boulay A, Quevarec E, Malet I, Nicastro G, Chamontin C, Perrin S, Henriquet C, Pugnière M, Courgnaud V, Blaise M, Marcelin AG, Taylor IA, Chaloin L, Arhel NJ. A new class of capsid-targeting inhibitors that specifically block HIV-1nuclear import. EMBO Mol Med. 2024 Nov;16(11):2918-2945.
Feuillard J, Couston J, Benito Y, Hodille E, Dumitrescu O, Blaise M*. Biochemical and structural characterization of a class A β-lactamase from Nocardia cyriacigeorgica. Acta Crystallogr F Struct Biol Commun. 2024 Jan 1;80(Pt 1):13-21.
Paris T, Kiss A, Signor L, Lutfalla G, Blaise M, Boeri Erba E, Chaloin L, Yatime L. The IbeA protein from adherent invasive Escherichia coli is a flavoprotein sharing structural homology with FAD-dependent oxidoreductases. FEBS J. 2024 Jan;291(1):177-203.
2023
Marty L, Pastre C, Blaise M, Beaumelle B. Mécanisme d’association de la protéine Tat du VIH à la particule virale [Mechanism enabling HIV Tat association with viral particles]. Med Sci (Paris). 2023 Dec;39(12):915-917.
Couston J, Guo Z, Wang K, Gourdon P*, Blaise M*. Cryo-EM structure of the trehalose monomycolate transporter, MmpL3, reconstituted into peptidiscs. Curr Res Struct Biol. 2023 Nov 8;6:100109.
Ung KL, Poussineau C, Couston J, Alsarraf HMAB, Blaise M*. Crystal structure of MAB_4123, a putative flavin-dependent monooxygenase from Mycobacterium abscessus. Acta Crystallogr F Struct Biol Commun. 2023 May 1;79(Pt 5):128-136.
Schatz M, Marty L, Ounadjela C, Tong PBV, Cardace I, Mettling C, Milhiet PE, Costa L, Godefroy C, Pugnière M, Guichou JF, Mesnard JM, Blaise M, Beaumelle B. A Tripartite Complex HIV-1 Tat-Cyclophilin A-Capsid Protein Enables Tat Encapsidation That Is Required for HIV-1 Infectivity. J Virol. 2023 Apr 27;97(4):e0027823.
McKellar J, Arnaud-Arnould M, Chaloin L, Tauziet M, Arpin-André C, Pourcelot O, Blaise M, Moncorgé O, Goujon C. An evolutionarily conserved N-terminal leucine is essential for MX1 GTPase antiviral activity against different families of RNA viruses. J Biol Chem. 2023 Jan;299(1):102747.
2022
Bonaventure B, Rebendenne A, Chaves Valadão AL, Arnaud-Arnould M, Gracias S, Garcia de Gracia F, McKellar J, Labaronne E, Tauziet M, Vivet-Boudou V, Bernard E, Briant L, Gros N, Djilli W, Courgnaud V, Parrinello H, Rialle S, Blaise M, Lacroix L, Lavigne M, Paillart JC, Ricci EP, Schulz R, Jouvenet N, Moncorgé O, Goujon C. The DEAD box RNA helicase DDX42 is an intrinsic inhibitor of positive-strand RNA viruses. EMBO Rep. 2022 Nov 7;23(11):e54061.
Alsarraf HMAB, Ung KL, Johansen MD, Dimon J, Olieric V, Kremer L, Blaise M*. Biochemical, structural, and functional studies reveal that MAB_4324c from Mycobacterium abscessus is an active tandem repeat N-acetyltransferase. FEBS Lett. 2022 Jun;596(12):1516-1532.
Ung KL, Alsarraf H, Kremer L, Blaise M*. MmpL3, the trehalose monomycolate transporter, is stable in solution in several detergents and can be reconstituted into peptidiscs. Protein Expr Purif. 2022 Mar;191:106014.
2021
Gysel K, Laursen M, Thygesen MB, Lironi D, Bozsóki Z, Hjuler CT, Maolanon NN, Cheng J, Bjørk PK, Vinther M, Madsen LH, Rübsam H, Muszyński A, Ghodrati A, Azadi P, Sullivan JT, Ronson CW, Jensen KJ, Blaise M, Radutoiu S, Stougaard J, Andersen KR. Kinetic proofreading of lipochitooligosaccharides determines signal activation of symbiotic plant receptors. Proc Natl Acad Sci U S A. 2021 Nov 2;118(44):e2111031118.
Arnaud-Arnould M, Tauziet M, Moncorgé O, Goujon C, Blaise M*. Crystal structure of the TLDc domain of human NCOA7-AS. Acta Crystallogr F Struct Biol Commun. 2021 Aug 1;77(Pt 8):230-237.
2020
Küssau T, Van Wyk N, Johansen MD, Alsarraf HMAB, Neyret A, Hamela C, Sørensen KK, Thygesen MB, Beauvineau C, Kremer L, Blaise M*. Functional Characterization of the <i>N</i>-Acetylmuramyl-l-Alanine Amidase, Ami1, from <i>Mycobacterium abscessus</i>. Cells. 2020 Nov 4;9(11):2410.
Ung KL, Kremer L, Blaise M*. Structural analysis of the N-acetyltransferase Eis1 from Mycobacterium abscessus reveals the molecular determinants of its incapacity to modify aminoglycosides. Proteins. 2020 Jan;89(1):94-106
Wong JEMM*, Blaise M*. Report of false positives when using zymography to assess peptidoglycan hydrolytic activity of an endopeptidase with multiple LysM domains. Biochimie. 2020. Oct;177:25-29.
Bozsoki Z, Gysel K, Hansen SB, Lironi D, Krönauer C, Feng F, de Jong N, Vinther M, Kamble M, Thygesen MB, Engholm E, Kofoed C, Fort S, Sullivan JT, Ronson CW, Jensen KJ, Blaise M, Oldroyd G, Stougaard J, Andersen KR, Radutoiu S. Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity. Science. 2020. Aug 7;369(6504):663-670.
Faïon L, Djaout K, Frita R, Pintiala C, Cantrelle FX, Moune M, Vandeputte A, Bourbiaux K, Piveteau C, Herledan A, Biela A, Leroux F, Kremer L, Blaise M, Tanina A, Wintjens R, Hanoulle X, Déprez B, Willand N, Baulard AR, Flipo M. Discovery of the first Mycobacterium tuberculosis MabA (FabG1) inhibitors through a fragment-based screening. Eur J Med Chem. 2020 Aug 15;200:112440.
de Ruyck J, Dupont C, Lamy E, Le Moigne V, Biot C, Guérardel Y, Herrmann JL, Blaise M, Grassin-Delyle S, Kremer L, Dubar F. Structure-Based Design and Synthesis of Piperidinol-Containing Molecules as New <i>Mycobacterium abscessus</i> Inhibitors. Chemistry Open. 2020 Mar 20;9(3):351-365.
Blaise M, Kremer L. Self-control of vitamin K<sub>2</sub> production captured in the crystal. J Biol Chem. 2020 Mar 20;295(12):3771-3772.