2024
Cyclophostin and Cyclipostins analogues counteract macrolide-induced resistance mediated by Erm(41) in Mycobacterium abscessus.
M. Sarrazin, I. Poncin, P. Fourquet, S. Audebert, L. Camoin, Y. Denis, P. Santucci, C. D. Spilling, L. Kremer, V. Le Moigne, J.‑Louis Herrmann, J.-F. Cavalier, and S. Canaan. 2024. J. Biomed. Sci. In Press.
SQ31f is a potent non-tuberculous mycobacteria antibiotic by targeting specifically the mycobacterial F-ATP synthase.
P. Ragunathan, P. Sae-Lao, A. Harikishore, W. Daher, F. Roquet-Banères, L. Kremer, R. W. Bates, and G. Grüber. 2024. J. Antimicrob. Chemother. In Press.
Trehalose polyphleates participate in Mycobacterium abscessus fitness and pathogenesis.
S. Malmsheimer, W. Daher, Y. Tasrini, C. Hamela, J. J. Aguilera-Correa, C. Chalut, G. F. Hatfull and L. Kremer. 2024. mBio. 30: e0297024. DOI: 10.1128/mbio.02970-24.
A dTDP-L-Rhamnose 4-epimerase required for glycopeptidolipid biosynthesis in Mycobacterium abscessus.
J. J. Aguilera-Correa, F. Wei, L.-D. Leclercq, Y. Tasrini, E. Mullapudi, W. Daher, K. Nakajima, S. Canaan, J.-L. Herrmann, M. Wilmanns, Y. Guérardel, L. Wen, and L. Kremer. 2024. J. Biol. Chem. 300: 107852. DOI: 10.1016/j.jbc.2024.107852.
Combination of serological and cytokine release assays for improved diagnosis of childhood tuberculosis in Zambia (PROMISE-TB).
E. Tuaillon, M. Mwyia, K. Bollore, A. Pisoni, P.-A. Rubbo, M. Richard, L. Kremer, M. M.W. Tonga, D. M. Chanda, M. Peries, R. Vallo, S. Eymard-Duvernay, M. D’Ottavi, C. Kankasa, P. Van de Perre, J.-P. Moles, and N. Nagot. 2024. Int. J. Infect. Dis. 148: 107248. DOI: 10.1016/j.ijid.2024.107248.
Rational design and microwave-promoted synthesis of triclosan-based dimers: Targeting InhA for anti-mycobacterial profiling.
Shekhar, F. Roquet-Banères, A. Anand, L. Kremer, and V. Kumar. 2024. R. Soc. Open Sci. 11: 240676. DOI: 10.1098/rsos.240676.
The diversity of clinical Mycobacterium abscessus isolates in morphology, glycopeptidolipids and infection rates in a macrophage model.
V. Pichler, L. Dalkilic, G. Shoaib, T. Shapira, L. Rankine-Wilson, Y.-M. Boudehen, J. D. Chao, D. Sexton, M. Prieto, B. S. Quon, E. I. Tocheva, L. Kremer, W. Hsiao, and Yossef Av-Gay. 2024. J. Med. Microbiol. 73: 001869. DOI: 10.1099/jmm.0.001869.
Cu-promoted synthesis of triclosan-Mannich and Glaser adducts: anti-mycobacterial evaluation with in silico validations.
Shekhar, M. Alcaraz, A. Anand, R. K. Sharma, L. Kremer, and V. Kumar. 2024. Future Med. Chem. 16: 949-961. DOI: 10.4155/fmc-2023-0298.
Evaluation and activity of new porphyrin-peptide cage-type conjugates for the photoinactivation of Mycobacterium abscessus.
M. Alcaraz, S. Lyonnais, C. Ghosh, J. Aguilera-Correa, S. Richeter, S. Ulrich, and L. Kremer. 2024. mSpectrum. 12: e0000624. DOI: 10.1128/spectrum.00006-24.
Loss of LpqM proteins in Mycobacterium abscessus is associated with impaired intramacrophage survival.
Y.-M. Boudehen, W. Daher, F. Roquet-Baneres, and L. Kremer. 2024. mSpectrum. 12: e0383723. DOI: 10.1128/spectrum.03837-23.
High efficacy of the F-ATP synthase inhibitor TBAJ-5307 against non-tuberculous mycobacteria in vitro and in vivo.
P. Ragunathan, P. Sae-Lao, C. Hamela, M. Alcaraz, A. Krah, W. Han Poh, C. Jia Ern Pee, A. Yick Hou Lim, S. A. Rice, K. Pethe, P. John Bond, T. Dick, L. Kremer*, R. W. Bates*, and G. Grüber*. 2024. J. Biol. Chem. 300:105618. *Co-last corresponding authors.
Modeling non-tuberculous mycobacterial infections in zebrafish.
D. Johansen, H. P. Spaink, S. H. Oehlers, and L. Kremer. 2024. Trends Microbiol. S0966-842X(23)00329-3.
2023
Mycobacterium abscessus, a complex of three fast-growing subspecies sharing virulence traits with slow-growing mycobacteria.
Lagune, L. Kremer, and J.-L. Herrmann. 2023. Clin. Microbiol. Infect. S1198-743X(23)00485-8.
Tailoring selective triclosan azo-adducts: Design, synthesis, and anti-mycobacterial evaluation.
Shekhar, M. Alcaraz, P. Seboletswe, N. Manhas, L. Kremer, P. Singh, and V. Kumar. 2023. Heliyon. 9: e22182.
Intrabacterial lipid inclusion-associated proteins: A core machinery conserved from saprophyte Actinobacteria to the human pathogen Mycobacterium tuberculosis.
Dargham, I. Mallick1, L. Kremer, P. Santucci, and S. Canaan. 2023. FEBS Open Bio. 13: 2306-2323.
Silencing essential gene expression in Mycobacterium abscessus during infection.
Y.-M. Boudehen, Y. Tasrini, J. J. Aguilera-Correa, M. Alcaraz, and L. Kremer. 2023. mSpectrum. 13: e0283623.
Characterization of Mycobacterium abscessus colony-biofilms based on bi-dimensional images.
J. J. Aguilera-Correa, Y.-M. Boudehen, and L. Kremer. 2023. Antimicrob. Agents Chemother. 67: e0040223.
Therapeutically useful mycobacteriophages BPs and Muddy require trehalose polyphleates.
K. S. Wetzel, M. Illouz, L. Abad, H. G. Aull, D. A. Russell, R. Garlena, M. Cristinziano, S. Malmsheimer, C. Chalut, G. F. Hatfull*, and L. Kremer*. 2023. Nat. Microbiol. 8: 1717-1731 *Co-last corresponding authors.
Multiple Mycobacterium abscessus O-acetyltransferases influence glycopeptidolipid structure and colony morphotype.
M. Illouz, L.-D. Leclercq, C. Dessenne, G. Hatfull, W. Daher, L. Kremer*, and Y. Guérardel*. 2023. J. Biol. Chem. 299: 104979. *Co-last corresponding authors.
New therapeutic strategies for Mycobacterium abscessus pulmonary diseases – Untapping the mycolic acid pathway.
M. Alcaraz, T. E. Edwards, and L. Kremer. 2023. Expert Rev. Anti. Infect. Ther. 21: 813-829.
In vitro and in vivo efficacy of NITD-916 against Mycobacterium fortuitum.
F. Roquet-Banères, M. Alcaraz, C. Hamela, J. Abendroth, T. E. Edwards, and L. Kremer. 2023. Antimicrob. Agents Chemother. e01607-22.
Mycobacterial biotin biosynthesis counters airway alkalinity. W. Daher, and L. Kremer. 2023. Nat. Microbiol. 8: 369-370.
Mycobacterium abscessus alkylhydroperoxide reductase C promotes cell invasion by binding to tetraspanin CD81. J. Karam, F. P. Blanchet, E. Vivès, P. Boisguérin, Y.-M. Boudehen, L. Kremer*, and W. Daher*. 2023. iScience. 26, 106042. *Co-last corresponding authors.
Human IRF1 governs macrophagic IFN-γ immunity to mycobacteria.
J. Rosain, A.-L. Neehus, J. Manry, R. Yang, J. Le Pen, W. Daher, Z. Liu, Y.-H. Chan, N. Tahuil, Ö. Türel, M. Bourgey, M. Ogishi, J.-M. Doisne, H. M. Izquierdo, T. Shirasaki, T. Le Voyer, A. Guérin, P. Bastard, M. Moncada-Velez, J. E. Han, T. Khan, F. Rapaport, S.-H. Hong, A. Cheung, K. Haake, B. C. Mindt, L. Perez, Q. Philippot, D. Lee, P. Zhang, D. Rinchai, F. Al Ali, M. M. A. Ata, M. Rahman, J. N. Peel, S. Heissel, H. Molina, Y. Kendir-Demirkol, R. Bailey, S. Zhao, J. Bohlen, M. Mancini, Y. Seeleuthner, M. Roelens, L. Lorenzo, C. Soudée, M. E. J. Paz, M. L. Gonzalez, M. Jeljeli, J. Soulier, S. Romana, A.-S. L’Honneur, M. Materna, R. Martínez-Barricarte, M. Pochon, C. Oleaga-Quintas, A. Michev, M. Migaud, R. Lévy, M.-A. Alyanakian, F. Rozenberg, C. A. Croft, G. Vogt, J.-F. Emile, L. Kremer, C. S. Ma, J. H. Fritz, S. Lemon, A. N. Spaan, N. Manel, L. Abel, M. R. MacDonald, S. Boisson-Dupuis, N. Marr, S. G. Tangye, J. P. Di Santo, Q. Zhang, S.-Y. Zhang, C. M. Rice, V. Béziat, N. Lachmann, D. Langlais, J.-L. Casanova, P. Gros, and J. Bustamante. 2023. Cell. 186: 1-25.
Synthesis and testing of analogs of the tuberculosis drug SQ109 against bacteria and protozoa: Identification of lead compounds against Mycobacterium abscessus and malaria parasites.
M. Stampolaki, S. R. Malwal, N. Alvarez-Cabrera, Z. Gao, M. Moniruzzaman, S. O. Babii, N. Naziris, A. Rey-Cibati, M. Valladares-Delgado, A. L. Turcu, K.-H. Baek, T.-N. Phan, H. Lee, M. Alcaraz, S. Watson, M. van der Watt, D. Coertzen, N. Efstathiou, M. Chountoulesi, C. M. Shoen, Ioannis P. Papanastasiou, J. Brea, M. H. Cynamon, L.-M. Birkholtz, L. Kremer, J. H. No, S. Vázquez, G. Benaim, C. Demetzos, H. I. Zgurskaya, T. Dick, E. Oldfield, and A. D. Kolocouris. 2023. ACS Infect. Dis. 9: 342-364.
The molecular basis and downstream immune consequences of mycobacteria-host cell interactions.
W. Daher, V. Pichler, J. Karam, O. Neyrolles, and L. Kremer. 2023. The molecular basis and downstream immune consequences of mycobacteria-host cell interactions. FEMS Microbiol. Rev. 47: fuad009.
2022
Tricyclic SpiroLactams kill mycobacteria in vitro and in vivo by inhibiting type II NADH dehydrogenases.
S. Dam, S. Tangara, C. Hamela, T. Hattabi, L. Faïon, P. Carre, R. Antoine, A. Herledan, F. Leroux, C. Piveteau, M. Eveque, M. Flipo, B. Deprez, L. Kremer, N. Willand, B. Villemagne, and R.C. Hartkoorn. 2022. J. Med. Chem. 65: 16651-16664.
Amikacin Liposomal Inhalation Suspension in the treatment of Mycobacterium abscessus lung infection: a French observational experience.
R. Chiron, W. Hoefsloot, J. van Ingen, H. Marchandin, L. Kremer, H. Morisse-Pradier, J. Charriot, J.-P Mallet, J.-L. Herrmann, D. Caimmi, J. Moreau, Y. Dumont, S. Godreuil, A. Bergeron, M. Drevait, E. Bouzat-Rossigneux, N. Terrail, C. Andrejak, N. Veziris, D. Grenet, A. Coudrat, and E. Catherinot. 2022. Open Forum Infect. Dis. 9: ofac465.
Efficacy and Mode of Action of a Direct Inhibitor of Mycobacterium abscessus InhA.
Alcaraz M, Roquet-Banères F, Leon-Icaza SA, Abendroth J, Boudehen YM, Cougoule C, Edwards TE, Kremer L. ACS Infect Dis. 2022 Oct 14;8(10):2171-2186. doi: 10.1021/acsinfecdis.2c00314. Epub 2022 Sep 15. PMID: 3610799
C25-modified rifamycin derivatives with improved activity against Mycobacterium abscessus. L. Paulowski, K. Beckham, M. D. Johansen, L. Berneking, N. Van, Y. Degefu, S. Staack, F. Vasquez Sotomayor, L. Asar, H. Rohde, B. B. Aldridge, M. Aepfelbacher, A. Parret, M. Wilmanns, L. Kremer, K. Combrink, F. P. Maurer. 2022. PNAS Nexus. 1: pgac130.
Intrabacterial lipid inclusions: overview of an amazing organelle. In: Biology of Mycobacterial Lipids.
Dargham, I. Mallick, D. Raze, L. Kremer, and S. Canaan. 2022. Academic Press. Edited by Z. Fatima & S. Canaan. 253-269.
Designing quinoline-isoniazid hybrids as potent anti-tubercular agents inhibiting mycolic acid biosynthesis.
Alcaraz, B. Sharma, F. Roquet-Banères, C. Conde, T. Cochard, F. Biet, V. Kumar, and L. Kremer. 2022. Eur. J. Med. Chem. 239: 114531.
The ESX-4 substrates, EsxU and EsxT, modulate Mycobacterium abscessus fitness.
Lagune, V. Le Moigne, M. D. Johansen, F. V. Sotomayor, W. Daher, C. Petit, G. Cosentino, L. Paulowski, T. Gutsmann, M. Wilmanns, F. P. Maurer, J.-L. Herrmann, F. Girard-Misguich, and L. Kremer. 2022. PLOS Pathog. 18: e1010771.
Biochemical, structural and functional studies reveal that MAB-4324c from Mycobacterium abscessus is an active tandem repeat N-acetyltransferase.
H. M. A. B. Alsarraf, K. L. Ung, M. D. Johansen, J. Dimon, V. Olieric, L. Kremer, and M. Blaise. 2022. FEBS Lett. 596: 1516-1532.
Exploring macrophage-dependent wound regeneration during mycobacterial infection in zebrafish.
C. Bohaud, M. D. Johansen, B. Varga, R. Contreras, A. Barthelaix, C. Hamela, D. Sapède, T. Cloitre, C. Gergely, C. Jorgensen, L. Kremer, and F. Djouad. 2022. Front. Immunol. 13: 838425.
Glycopeptidolipid glycosylation controls surface properties and pathogenicity in Mycobacterium abscessus.
W. Daher, L.-D. Leclercq, M. D. Johansen, C. Hamela, J. Karam, X. Trivelli, J. Nigou, Y. Guérardel, and L. Kremer. 2022. Cell Chem. Biol. 29: 1-15.
Rough and smooth variant Mycobacterium abscessus are differentially controlled by host immunity during chronic infection of adult zebrafish.
J. Y. Kam, E. Hortle, E. Krogman, S. E. Warner, K. Wright, K. Luo, T. Cheng, P. M. Cholan, K. Kikuchi, J. A. Triccas, W. J. Britton, M. D. Johansen, L. Kremer, and S. H. Oehlers. 2022. Nat. Comm. 13: 952.
2021
Mycobacterium abscessus, un modèle de résistance aux différentes classes d’antibiotiques.
M. Illouz, M. Alcaraz, F. Roquet-Banères, and L. Kremer. 2021. Med. Sci. 37: 993-1001.
Intrabacterial lipid inclusions in mycobacteria: unexpected key players in survival and pathogenesis?
I. Mallick, P. Santucci, I. Poncin, V. Point, L. Kremer, J.-F. Cavalier, and S. Canaan. 2021. FEMS Microbiol. Rev. fuab029: 1-19.
MmpL3, the trehalose monomycolate transporter, is stable in solution in several detergents and can be reconstituted into peptidiscs
K. L. Ung, H. Alsarraf, L. Kremer, and M. Blaise. 2021. Protein Exp. Purif. 191: 106014.
1H-1,2,3-triazole embedded Isatin-Benzaldehyde-bis(heteronuclearhydrazones): design, synthesis, antimycobacterial, and cytotoxic evaluation
B. Sharma, S. Kumar, Preeti, M. D. Johansen, L. Kremer, and V. Kumar. 2021. Chem. Biol. Drug Des. 00:1-7.
Efficacy of epetraborole against Mycobacterium abscessus is increased with norvaline
J. R. Sullivan, A. Lupien, E. Kalthoff, C. Hamela, L. Taylor, K. A. Munro, T. M. Schmeing, L. Kremer, and M. A. Behr. 2021. PLOS Pathog. 17: e1009965.
Elimination of PknL and MSMEG_4242 in Mycobacterium smegmatis alters the character of the outer cell envelope and selects for mutations in Lsr2
E. Baez, L. Querales, C. A. Aranaga, G. López, E. Guerrero, L. Kremer, S. Carrère-Kremer, A. Viljoen, M. Daffé, F. Laval, S. Cole, A. Benjak, P. Alzari, G. André-Leroux, William Jacobs, Jr., C. Vilchéze, and H. Takiff. 2021. Cell Surf. 7: 100060.
Synthesis and biological evaluation of 3,4-dihydro-1H-[1,4] oxazepino [6,5,4-hi] indol-1-ones and 4,6-dihydrooxepino [5,4,3-cd] indol-1(3H)-ones as Mycobacterium tuberculosis inhibitors
Chamcieux, C. Raynaud, A. Viljoen, L. Chene, J. Thibonnet, S. P. Vincent, L. Kremer, and E. Thiery. 2021. Bioorg. Med. Chem. 43: 116248
Biological and Biochemical Evaluation of Isatin-Isoniazid Hybrids as Bactericidal Candidates against Mycobacterium tuberculosis
M. Johansen, Shalini, S. Kumar, C. Raynaud, D. H. Quan, W. J. Britton, P. M. Hansbro, V. Kumar, and L. Kremer. 2021. Antimicrobial. Agents. Chemother. 65: e00011-21.
The Role of Macrophages During Mammalian Tissue Remodeling and Regeneration Under Infectious and Non-Infectious Conditions
Bohaud, M. D. Johansen, C. Jorgensen, L. Kremer, N. Ipseiz, and F. Djouad. 2021. Front. Immunol. 12: 707856.
Conserved and specialized functions of Type VII secretion systems in non-tuberculous mycobacteria
Lagune, C. Petit, F. V. Sotomayor, M. D. Johansen, K. S. H. Beckham, C. Ritter, F. Girard-Misguich*, M. Wilmanns*, L. Kremer*, F. P. Maurer*, and J.-L. Herrmann*. 2021. Microbiology. 167: 001054. *Co-corresponding authors.
The Role of Macrophages During Zebrafish Injury and Tissue Regeneration Under Infectious and Non-Infectious Conditions
Bohaud, M. D. Johansen, C. Jorgensen, N. Ipseiz*, L. Kremer*, and F. Djouad*. 2021. Front. Immunol. 12: 707824. *Equal contribution.
Mycobacterium abscessus
Y-M. Boudehen, L. Kremer. Trends Microbiol. 2021 Jul 23;S0966-842X(21)00138-4. doi: 10.1016/j.tim.2021.06.006.
Mycobacteriophage-antibiotic therapy promotes enhanced clearance of drug-resistant Mycobacterium abscessus
D. Johansen, M. Alcaraz, R.M. Dedrick, F. Roquet-Banères, C. Hamela, G. F. Hatfull, and L. Kremer. 2021. Dis. Model. Mech. 14: dmm049159.
2020
Functional Characterization of the N-Acetylmuramyl-l-Alanine Amidase, Ami1, from Mycobacterium abscessus
Küssau T, Van Wyk N, Johansen MD, Alsarraf HMAB, Neyret A, Hamela C, Sørensen KK, Thygesen MB, Beauvineau C, Kremer L, Blaise M.Cells. 2020 Nov 4;9(11):2410. doi: 10.3390/cells9112410.
Design and synthesis of 4-Aminoquinoline-isoindoline-dione-isoniazid triads as potential anti-mycobacterials.
Rani A, Johansen MD, Roquet-Banères F, Kremer L, Awolade P, Ebenezer O, Singh P, Sumanjit, Kumar V. Bioorg Med Chem Lett. 2020 Sep 24;30(22):127576. doi: 10.1016/j.bmcl.2020.127576.
The roles of tetraspanins in bacterial infections.
Karam J, Méresse S, Kremer L, Daher W.
Cell Microbiol. 2020 Sep 9:e13260. doi: 10.1111/cmi.13260.
Structural analysis of the N-acetyltransferase Eis1 from Mycobacterium abscessus reveals the molecular determinants of its incapacity to modify aminoglycosides.
Ung KL, Kremer L, Blaise M.Proteins. 2020 Aug 28. doi: 10.1002/prot.25997.
O-Methylation of the glycopeptidolipid acyl chain defines surface hydrophobicity of Mycobacterium abscessus and macrophage invasion.
Daher W, Leclercq LD, Viljoen A, Karam J, Dufrêne YF, Guerardel Y, Kremer L.
ACS Infect Dis. 2020 Aug 28. doi: 10.1021/acsinfecdis.0c00490.
CFTR Depletion Confers Hypersusceptibility to Mycobacterium fortuitum in a Zebrafish Model.
Johansen MD, Kremer L.Front Cell Infect Microbiol. 2020 Jul 17;10:357. doi: 10.3389/fcimb.2020.00357. eCollection 2020. PMID: 32850470
Rifabutin is bactericidal against intracellular and extracellular forms of Mycobacterium abscessus.
Johansen MD, Daher W, Roquet-Banères F, Raynaud C, Alcaraz M, Maurer FP, Kremer L.Antimicrob Agents Chemother. 2020 Aug 17:AAC.00363-20. doi: 10.1128/AAC.00363-20.
Synthesis and evaluation of heterocycle structures as potential inhibitors of Mycobacterium tuberculosis UGM.
Maaliki C, Fu J, Villaume S, Viljoen A, Raynaud C, Hammoud S, Thibonnet J, Kremer L, Vincent SP, Thiery E.
Bioorg Med Chem. 2020 Jul 1;28(13):115579. doi: 10.1016/j.bmc.2020.115579.
Discovery of the first Mycobacterium tuberculosis MabA (FabG1) inhibitors through a fragment-based screening.
Faïon L, Djaout K, Frita R, Pintiala C, Cantrelle FX, Moune M, Vandeputte A, Bourbiaux K, Piveteau C, Herledan A, Biela A, Leroux F, Kremer L, Blaise M, Tanina A, Wintjens R, Hanoulle X, Déprez B, Willand N, Baulard AR, Flipo M.
Eur J Med Chem. 2020 May 18;200:112440. doi: 10.1016/j.ejmech.2020.112440.
Fast chemical force microscopy demonstrates that glycopeptidolipids define nanodomains of varying hydrophobicity on mycobacteria.
Viljoen A, Viela F, Kremer L, Dufrêne YF.
Nanoscale Horiz. 2020 Apr 21. doi: 10.1039/c9nh00736a.
A zebrafish model of Mycobacterium kansasii infection reveals large extracellular cord formation.
Johansen MD, Kremer L.
J Infect Dis. 2020 Apr 17:jiaa187. doi: 10.1093/infdis/jiaa187
Efficacy of bedaquiline, alone or in combination with imipenem, against Mycobacterium abscessus in C3HeB/FeJ mice.
Le Moigne V, Raynaud C, Moreau F, Dupont C, Nigou J, Neyrolles O, Kremer L, Herrmann JL.
Antimicrob Agents Chemother. 2020 Apr 6:AAC.00114-20. doi:10.1128/AAC.00114-20
Self-control of vitamin K2 production captured in the crystal.
Blaise M, Kremer L.
J Biol Chem. 2020 Mar 20;295(12):3771-3772. doi: 10.1074/jbc.H120.013113.
Structure-Based Design and Synthesis of Piperidinol-Containing Molecules as New Mycobacterium abscessus Inhibitors.
de Ruyck J, Dupont C, Lamy E, Le Moigne V, Biot C, Guérardel Y, Herrmann JL, Blaise M, Grassin-Delyle S, Kremer L, Dubar F.
ChemistryOpen. 2020 Mar 20;9(3):351-365. doi: 10.1002/open.202000042.
The endogenous galactofuranosidase GlfH1 hydrolyzes mycobacterial arabinogalactan.
Shen L, Viljoen A, Villaume S, Joe M, Halloum I, Chêne LP, Méry A, Fabre E, Takegawa K, Lowary TL, Vincent SP, Kremer L, Guérardel Y, Mariller C.
J Biol Chem. 2020 Feb 27. pii: jbc.RA119.011817. doi: 10.1074/jbc.RA119.011817.
Non-tuberculous mycobacteria and the rise of Mycobacterium abscessus.
Johansen MD, Herrmann JL, Kremer L.
Nat Rev Microbiol. 2020 Feb 21. doi: 10.1038/s41579-020-0331-1.
Synergistic interactions of indole-2-carboxamides and β-lactam antibiotics against Mycobacterium abscessus.
Raynaud C, Daher W, Roquet-Banères F, Johansen MD, Stec J, Onajole OK, Ordway D, Kozikowski AP, Kremer L.
Antimicrob Agents Chemother 2020 Feb 10. pii: AAC.02548-19. doi: 10.1128/AAC.02548-19.
Active Benzimidazole Derivatives Targeting the MmpL3 Transporter in Mycobacterium abscessus.
Raynaud C, Daher W, Johansen MD, Roquet-Banères F, Blaise M, Onajole OK, Kozikowski AP, Herrmann JL, Dziadek J, Gobis K, Kremer L.
ACS infectious diseases ACS Infect Dis 2020 Jan 7. doi: 10.1021/acsinfecdis.9b00389.
2019
The crystal structure of the mycobacterial trehalose monomycolate transport factor A, TtfA, reveals an atypical fold.
Ung KL, Alsarraf HMAB, Kremer L, Blaise M.
Proteins 2019 Dec 12. doi: 10.1002/prot.25863.
Dissecting erm(41)-mediated macrolide inducible resistance in Mycobacterium abscessus.
Richard M, Gutiérrez AV, Kremer L.
Antimicrobial agents and chemotherapy 2019 Dec 2. pii: AAC.01879-19. doi: 10.1128/AAC.01879-19.
A piperidinol-containing molecule is active against Mycobacterium tuberculosis by inhibiting the mycolic acid flippase activity of MmpL3.<
Dupont C, Chen Y, Xu Z, Roquet-Banères F, Blaise M, Witt AK, Dubar F, Biot C, Guérardel Y, Maurer FP, Chng SS, Kremer L.
The Journal of biological chemistry>J Biol Chem. 2019 Nov 15;294(46):17512-17523. doi: 10.1074/jbc.RA119.010135.
Variedly connected 1,8-naphthalimide-7-chloroquinoline conjugates: Synthesis, anti-mycobacterial and cytotoxic evaluation.
Shalini, Johansen MD, Kremer L, Kumar V.
Bioorganic chemistry 2019 Nov;92:103241. doi: 10.1016/j.bioorg.2019.103241.
The TetR-family transcription factor MAB_2299c regulates the expression of two distinct MmpS-MmpL efflux pumps involved in cross-resistance to clofazimine and bedaquiline in Mycobacterium abscessus.
Gutiérrez AV, Richard M, Roquet-Banères F, Viljoen A, Kremer L.
Antimicrobial agents and chemotherapy 2019 Jul 22. pii: AAC.01000-19. doi: 10.1128/AAC.01000-19.
1H-benzo[d]imidazole derivatives affect MmpL3 in Mycobacterium tuberculosis.
Korycka-Machała M, Viljoen A, Pawełczyk J, Borówka P, Dziadek B, Gobis K, Brzostek A, Kawka M, Blaise M, Strapagiel D, Kremer L, Dziadek J.
Antimicrobial agents and chemotherapy>. 2019 Jul 22. pii: AAC.00441-19. doi: 10.1128/AAC.00441-19.
Cyclipostins and Cyclophostin analogs are multi-target inhibitors that impair growth of Mycobacterium abscessus.
Madani A, Ridenour JN, Martin BP, Paudel RR, Abdul Basir A, Le Moigne V, Herrmann JL, Audebert S, Camoin L, Kremer L, Spilling CD, Canaan S, Cavalier JF.
ACS infectious diseases. 2019 Jul 12. doi: 10.1021/acsinfecdis.9b00172.
Detection of Mycobacterium tuberculosis in paucibacillary sputum: performances of the Xpert MTB/RIF ultra compared to the Xpert MTB/RIF, and IS6110 PCR.
Kolia-Diafouka P, Carrère-Kremer S, Lounnas M, Bourdin A, Kremer L, Van de Perre P, Godreuil S, Tuaillon E.
Diagnostic microbiology and infectious disease. 2019 Aug;94(4):365-370. doi: 10.1016/j.diagmicrobio.2019.02.008.
Crystal structure of the aminoglycosides N-acetyltransferase Eis2 from Mycobacterium abscessus.
Ung KL, Alsarraf HMAB, Olieric V, Kremer L, Blaise M.
The FEBS journal 2019 Jun 29. doi: 10.1111/febs.14975.
Improved activity of bedaquiline by verapamil against Mycobacterium abscessus in vitro and in macrophages.
Viljoen A, Raynaud C, Johansen MD, Roquet-Banères F, Herrmann JL, Daher W, Kremer L.
Antimicrobial agents and chemotherapy. 2019 Jun 17. pii: AAC.00705-19. doi: 10.1128/AAC.00705-19.
Nitrogen deprivation induces triacylglycerol accumulation, drug tolerance and hypervirulence in mycobacteria.<
Santucci P, Johansen MD, Point V, Poncin I, Viljoen A, Cavalier JF, Kremer L, Canaan S.
Scientific reports. 2019 Jun 17;9(1):8667. doi: 10.1038/s41598-019-45164-5.
Lsr2 Is an Important Determinant of Intracellular Growth and Virulence in Mycobacterium abscessus.
Le Moigne V, Bernut A, Cortès M, Viljoen A, Dupont C, Pawlik A, Gaillard JL, Misguich F, Crémazy F, Kremer L, Herrmann JL.
Frontiers in microbiology. 2019 Apr 30;10:905. doi: 10.3389/fmicb.2019.00905.
Dissecting the membrane lipid binding properties and lipase activity of Mycobacterium tuberculosis LipY domains.
Santucci P, Smichi N, Diomandé S, Poncin I, Point V, Gaussier H, Cavalier JF, Kremer L, Canaan S.
The FEBS journal. 2019 Apr 29. doi: 10.1111/febs.14864.
Detection of Mycobacterium tuberculosis in paucibacillary sputum: performances of the Xpert MTB/RIF ultra compared to the Xpert MTB/RIF, and IS6110 PCR.
Kolia-Diafouka P, Carrère-Kremer S, Lounnas M, Bourdin A, Kremer L, Van de Perre P, Godreuil S, Tuaillon E.
Diagnostic microbiology and infectious disease. 2019 Feb 21. pii: S0732-8893(18)30545-5. doi: 10.1016/j.diagmicrobio.2019.02.008.
Design, Synthesis, Anti-mycobacterial and Cytotoxic evaluation of C-4 functionalized 1,8-naphthalimide-heterocyclic hydrazide conjugates.
Shalini, Johansen MD, Kremer L, Kumar V.
Chemical biology & drug design. 2019 Feb 18. doi: 10.1111/cbdd.13503.
CFTR Protects against Mycobacterium abscessus Infection by Fine-Tuning Host Oxidative Defenses.
Bernut A, Dupont C, Ogryzko NV, Neyret A, Herrmann JL, Floto RA, Renshaw SA, Kremer L.
Cell reports. 2019 Feb 12;26(7):1828-1840.e4. doi: 10.1016/j.celrep.2019.01.071.
2018
The most ancestral mycobacterial ESX-4 secretion system is essential for intracellular growth of Mycobacterium abscessus within environmental and human phagocytes].
Girard-Misguich F, Laencina L, Dubois V, Le Moigne V, Kremer L, Maljessi L, Brosch R, Herrmann JL.
Medecine sciences : M/S. 2018 Oct;34(10):795-797. doi: 10.1051/medsci/2018196.
Optimized Lysis-Extraction Method Combined With IS6110-Amplification for Detection of Mycobacterium tuberculosis in Paucibacillary Sputum Specimens.
Kolia-Diafouka P, Godreuil S, Bourdin A, Carrère-Kremer S, Kremer L, Van de Perre P, Tuaillon E.
Frontiers in microbiology. 2018 Sep 25;9:2224. doi: 10.3389/fmicb.2018.02224.
Mutations in the MAB_2299c TetR regulator confer cross-resistance to clofazimine and bedaquiline in Mycobacterium abscessus.
Richard M, Gutiérrez AV, Viljoen A, Rodriguez-Rincon D, Roquet-Baneres F, Blaise M, Everall I, Parkhill J, Floto RA, Kremer L. Antimicrobial agents and chemotherapy. 2018 Oct 15. pii: AAC.01316-18. doi: 10.1128/AAC.01316-18.
MmpL8MAB controls Mycobacterium abscessus virulence and production of a previously unknown glycolipid family.
Dubois V, Viljoen A, Laencina L, Le Moigne V, Bernut A, Dubar F, Blaise M, Gaillard JL, Guérardel Y, Kremer L, Herrmann JL, Girard-Misguich F.
Proceedings of the National Academy of Sciences of the United States of America »>Proc Natl Acad Sci U S A. 2018 Oct 9. pii: 201812984. doi: 10.1073/pnas.1812984115
Delineating the physiological roles of the PE and catalytic domain of LipY in lipid consumption in mycobacteria-infected foamy macrophages.
Santucci P, Diomandé S, Poncin I, Alibaud L, Viljoen A, Kremer L, de Chastellier C, Canaan S. Infection and immunity. 2018 Jul 9. pii: IAI.00394-18. doi: 10.1128/IAI.00394-18.
Glycopeptidolipids, a Double-Edged Sword of the Mycobacterium abscessus Complex.
Gutiérrez AV, Viljoen A, Ghigo E, Herrmann JL, Kremer L.
Frontiers in microbiology. 2018 Jun 5;9:1145. doi: 10.3389/fmicb.2018.01145. eCollection 2018. Review.
Structural rearrangements occurring upon cofactor binding in the Mycobacterium smegmatis β-ketoacyl-acyl carrier protein reductase MabA.
Küssau T, Flipo M, Van Wyk N, Viljoen A, Olieric V, Kremer L, Blaise M.
Acta Crystallogr D Struct Biol. 2018 May 1;74(Pt 5):383-393. doi:10.1107/S2059798318002917.
B cells response directed against Cut4 and CFP21 lipolytic enzymes in active and latent tuberculosis infections.
Rénier W, Bourdin A, Rubbo PA, Peries M, Dedieu L, Bendriss S, Kremer L, Canaan S, Terru D, Godreuil S, Nagot N, Van de Perre P, Tuaillon E.
PLoS One. 2018 Apr 30;13(4):e0196470. doi: 10.1371/journal.pone.0196470.
Mechanistic and Structural Insights Into the Unique TetR-Dependent Regulation of a Drug Efflux Pump in Mycobacterium abscessus.
Richard M, Gutiérrez AV, Viljoen AJ, Ghigo E, Blaise M, Kremer L.
Front Microbiol. 2018 Apr 5;9:649. doi: 10.3389/fmicb.2018.00649.
Neutrophil killing of Mycobacterium abscessus by intra- and extracellular mechanisms.
Malcolm KC, Caceres SM, Pohl K, Poch KR, Bernut A, Kremer L, Bratton DL, Herrmann JL, Nick JA.
PLoS One. 2018 Apr 19;13(4):e0196120. doi: 10.1371/journal.pone.0196120.
Alkylated/aminated nitroimidazoles and nitroimidazole-7-chloroquinoline conjugates: Synthesis and anti-mycobacterial evaluation.
Shalini, Viljoen A, Kremer L, Kumar V.
Bioorganic & medicinal chemistry letters. 2018 Mar 8. pii: S0960-894X(18)30200-2. doi:10.1016/j.bmcl.2018.03.021.
A Simple and Rapid Gene Disruption Strategy in Mycobacterium abscessus: On the Design and Application of Glycopeptidolipid Mutants.
Viljoen A, Gutiérrez AV, Dupont C, Ghigo E, Kremer L.
Frontiers in cellular and infection microbiology. 2018 Mar 14;8:69. doi: 10.3389/fcimb.2018.00069.
Identification of genes required for Mycobacterium abscessus growth in vivo with a prominent role of the ESX-4 locus.
Laencina L, Dubois V, Le Moigne V, Viljoen A, Majlessi L, Pritchard J, Bernut A, Piel L, Roux AL, Gaillard JL, Lombard B, Loew D, Rubin EJ, Brosch R, Kremer L, Herrmann JL, Girard-Misguich F.
Proceedings of the National Academy of Sciences of the United States of America. 2018 Jan 17. pii: 201713195. doi:10.1073/pnas.1713195115.
Cyclipostins and cyclophostin analogs inhibit the antigen 85C from Mycobacterium tuberculosis both in vitro and in vivo.
Viljoen A, Richard M, Nguyen PC, Fourquet P, Camoin L, Paudal RR, Gnawali GR, Spilling CD, Cavalier JF, Canaan S, Blaise M, Kremer L.
The Journal of biological chemistry. 2018 Feb 23;293(8):2755-2769. doi: 10.1074/jbc.RA117.000760. Epub 2018 Jan 4.
Cyclophostin and cyclipostins analogs, new promising molecules to treat mycobacterial-related diseases.
Nguyen PC, Madani A, Santucci P, Martin BP, Paudel RR, Delattre S, Herrmann JL, Spilling CD, Kremer L, Canaan S, Cavalier JF.
International journal of antimicrobial agents. 2017 Dec 11. pii: S0924-8579(17)30435-1. doi: 10.1016/j.ijantimicag.2017.12.001.
Severe inhibition of lipooligosaccharide synthesis induces TLR2-dependent elimination of Mycobacterium marinum from THP1-derived macrophages.
Szulc-Kielbik I, Pawelczyk J, Kielbik M, Kremer L, Dziadek J, Klink M.
Microbial cell factories. 2017 Nov 28;16(1):217. doi: 10.1186/s12934-017-0829-z.
Cyclipostins and Cyclophostin analogs as promising compounds in the fight against tuberculosis.
Nguyen PC, Delorme V, Bénarouche A, Martin BP, Paudel R, Gnawali GR, Madani A, Puppo R, Landry V, Kremer L, Brodin P, Spilling CD, Cavalier JF, Canaan S.
Sci Rep. 2017 Sep 18;7(1):11751. doi: 10.1038/s41598-017-11843-4.
Controlling Extra- and Intramacrophagic Mycobacterium abscessus by Targeting Mycolic Acid Transport.
Viljoen A, Herrmann JL, Onajole OK, Stec J, Kozikowski AP, Kremer L.
Front Cell Infect Microbiol. 2017 Sep 1;7:388. doi: 10.3389/fcimb.2017.00388. eCollection 2017.
Identification of inhibitors targeting Mycobacterium tuberculosis cell wall biosynthesis via dynamic combinatorial chemistry.
Fu J, Fu H, Dieu M, Halloum I, Kremer L, Xia Y, Pan W, Vincent SP.
Chem Commun (Camb). 2017 Sep 14. doi: 10.1039/c7cc05251k.
Azide-alkyne cycloaddition en route to 4-aminoquinoline-ferrocenylchalcone conjugates: synthesis and anti-TB evaluation.
Singh A, Viljoen A, Kremer L, Kumar V.
Future Med Chem. 2017 Sep 4. doi: 10.4155/fmc-2017-0098.
Bedaquiline inhibits the ATP synthase in Mycobacterium abscessus and is effective in infected zebrafish.
Dupont C, Viljoen A, Thomas S, Roquet-Banères F, Herrmann JL, Pethe K, Kremer L.
Antimicrob Agents Chemother. 2017 Aug 14. pii: AAC.01225-17. doi: 10.1128/AAC.01225-17.
Targeting Mycolic Acid Transport by Indole-2-carboxamides for the Treatment of Mycobacterium abscessus Infections.
Kozikowski AP, Onajole OK, Stec J, Dupont C, Viljoen A, Richard M, Chaira T, Lun S, Bishai W, Raj VS, Ordway D, Kremer L.
J Med Chem. 2017 Jul 13;60(13):5876-5888. doi: 10.1021/acs.jmedchem.7b00582.
Natural and Synthetic Flavonoids as Potent Mycobacterium tuberculosis UGM Inhibitors.
Villaume SA, Fu J, N’Go I, Liang H, Lou H, Kremer L, Pan W, Vincent SP.
Chemistry. 2017 Aug 1;23(43):10423-10429. doi: 10.1002/chem.201701812.
The Diverse Cellular and Animal Models to Decipher the Physiopathological Traits of Mycobacterium abscessus Infection.
Bernut A, Herrmann JL, Ordway D, Kremer L.
Front Cell Infect Microbiol. 2017 Apr 4;7:100. doi: 10.3389/fcimb.2017.00100. eCollection 2017. Review.
The diverse family of MmpL transporters in mycobacteria: from regulation to antimicrobial developments.
Viljoen A, Dubois V, Girard-Misguich F, Blaise M, Herrmann JL, Kremer L.
Mol Microbiol. 2017 Jun;104(6):889-904. doi: 10.1111/mmi.13675. Epub 2017 Apr 18. Review.
Acid-Fast Positive and Acid-Fast Negative Mycobacterium tuberculosis: The Koch Paradox.
Vilchèze C, Kremer L.
Microbiol Spectr. 2017 Mar;5(2). doi: 10.1128/microbiolspec.TBTB2-0003-2015. Review.
The influence of AccD5 on AccD6 carboxyltransferase essentiality in pathogenic and non-pathogenic Mycobacterium.
Pawelczyk J, Viljoen A, Kremer L, Dziadek J.
Sci Rep. 2017 Feb 16;7:42692. doi: 10.1038/srep42692.
Characterization of a mycobacterial cellulase and its impact on biofilm- and drug-induced cellulose production.
Van Wyk N, Navarro D, Blaise M, Berrin JG, Henrissat B, Drancourt M, Kremer L.
Glycobiology. 2017 May 1;27(5):392-399. doi: 10.1093/glycob/cwx014.
Binding of NADP+ triggers an open-to-closed transition in a mycobacterial FabG β-ketoacyl-ACP reductase.
Blaise M, Van Wyk N, Banères-Roquet F, Guérardel Y, Kremer L.
Biochem J. 2017 Mar 7;474(6):907-921. doi: 10.1042/BCJ20161052.
Resistance to Thiacetazone Derivatives Active against Mycobacterium abscessus Involves Mutations in the MmpL5 Transcriptional Repressor MAB_4384.
Halloum I, Viljoen A, Khanna V, Craig D, Bouchier C, Brosch R, Coxon G, Kremer L.
Antimicrob Agents Chemother. 2017 Mar 24;61(4). pii: e02509-16. doi: 10.1128/AAC.02509-16.
Inhibition of the β-Lactamase BlaMab by Avibactam Improves the In Vitro and In Vivo Efficacy of Imipenem against Mycobacterium abscessus.
Lefebvre AL, Le Moigne V, Bernut A, Veckerlé C, Compain F, Herrmann JL, Kremer L, Arthur M, Mainardi JL.
Antimicrob Agents Chemother. 2017 Mar 24;61(4). pii: e02440-16. doi: 10.1128/AAC.02440-16.
Current perspectives on the families of glycoside hydrolases of Mycobacterium tuberculosis: their importance and prospects for assigning function to unknowns.
van Wyk N, Drancourt M, Henrissat B, Kremer L.
Glycobiology. 2017 Jan;27(2):112-122. doi: 10.1093/glycob/cww099.
Attenuation of Mycobacterium species through direct and macrophage mediated pathway by unsymmetrical diaryl urea.
Velappan AB, Charan Raja MR, Datta D, Tsai YT, Halloum I, Wan B, Kremer L, Gramajo H, Franzblau SG, Kar Mahapatra S, Debnath J.
Eur J Med Chem. 2017 Jan 5;125:825-841. doi: 10.1016/j.ejmech.2016.09.083.
2016
The distinct fate of smooth and rough Mycobacterium abscessus variants inside macrophages.
Roux AL, Viljoen A, Bah A, Simeone R, Bernut A, Laencina L, Deramaudt T, Rottman M, Gaillard JL, Majlessi L, Brosch R, Girard-Misguich F, Vergne I, de Chastellier C, Kremer L, Herrmann JL.
Open Biol. 2016 Nov;6(11). pii: 160185.
Mycobacterium abscessus-Induced Granuloma Formation Is Strictly Dependent on TNF Signaling and Neutrophil Trafficking.
Bernut A, Nguyen-Chi M, Halloum I, Herrmann JL, Lutfalla G, Kremer L.
PLoS Pathog. 2016 Nov 2;12(11):e1005986. doi: 10.1371/journal.ppat.1005986. eCollection 2016
A unique PE_PGRS protein inhibiting host cell cytosolic defenses and sustaining full virulence of Mycobacterium marinum in multiple hosts.
Singh VK, Berry L, Bernut A, Singh S, Carrère-Kremer S, Viljoen A, Alibaud L, Majlessi L, Brosch R, Chaturvedi V, Geurtsen J, Drancourt M, Kremer L.
Cell Microbiol. 2016 Nov;18(11):1489-1507. doi: 10.1111/cmi.12606.
Experimental Models of Foamy Macrophages and Approaches for Dissecting the Mechanisms of Lipid Accumulation and Consumption during Dormancy and Reactivation of Tuberculosis.
Santucci P, Bouzid F, Smichi N, Poncin I, Kremer L, De Chastellier C, Drancourt M, Canaan S.
Front Cell Infect Microbiol. 2016 Oct 7;6:122. eCollection 2016. Review.
Identification of KasA as the cellular target of an anti-tubercular scaffold.
Abrahams KA, Chung CW, Ghidelli-Disse S, Rullas J, Rebollo-López MJ, Gurcha SS, Cox JA, Mendoza A, Jiménez-Navarro E, Martínez-Martínez MS, Neu M, Shillings A, Homes P, Argyrou A, Casanueva R, Loman NJ, Moynihan PJ, Lelièvre J, Selenski C, Axtman M, Kremer L, Bantscheff M, Angulo-Barturen I, Izquierdo MC, Cammack NC, Drewes G, Ballell L, Barros D, Besra GS, Bates RH.
Nat Commun. 2016 Sep 1;7:12581. doi: 10.1038/ncomms12581.
MAB_3551c encodes the primary triacylglycerol synthase involved in lipid accumulation in Mycobacterium abscessus.
Viljoen A, Blaise M, de Chastellier C, Kremer L.
Mol Microbiol. 2016 Nov;102(4):611-627. doi: 10.1111/mmi.13482.
MgtC as a Host-Induced Factor and Vaccine Candidate against Mycobacterium abscessus Infection.
Le Moigne V, Belon C, Goulard C, Accard G, Bernut A, Pitard B, Gaillard JL, Kremer L, Herrmann JL, Blanc-Potard AB.
Infect Immun. 2016 Sep 19;84(10):2895-903. doi: 10.1128/IAI.00359-16.
Deletion of a dehydratase important for intracellular growth and cording renders rough Mycobacterium abscessus avirulent.
Halloum I, Carrère-Kremer S, Blaise M, Viljoen A, Bernut A, Le Moigne V, Vilchèze C, Guérardel Y, Lutfalla G, Herrmann JL, Jacobs WR Jr, Kremer L.
Proc Natl Acad Sci U S A. 2016 Jul 19;113(29):E4228-37. doi: 10.1073/pnas.1605477113.
Mycobacterium lutetiense sp. nov., Mycobacterium montmartrense sp. nov. and Mycobacterium arcueilense sp. nov., members of a novel group of non-pigmented rapidly growing mycobacteria recovered from a water distribution system.
Konjek J, Souded S, Guerardel Y, Trivelli X, Bernut A, Kremer L, Welte B, Joyeux M, Dubrou S, Euzeby JP, Gaillard JL, Sapriel G, Heym B.
Int J Syst Evol Microbiol. 2016 Sep;66(9):3694-3702. doi: 10.1099/ijsem.0.001253.
A new piperidinol derivative targeting mycolic acid transport in Mycobacterium abscessus.
Dupont C, Viljoen A, Dubar F, Blaise M, Bernut A, Pawlik A, Bouchier C, Brosch R, Guérardel Y, Lelièvre J, Ballell L, Herrmann JL, Biot C, Kremer L.
Mol Microbiol. 2016 Aug;101(3):515-29. doi: 10.1111/mmi.13406.
Use of the Salmonella MgtR peptide as an antagonist of the Mycobacterium MgtC virulence factor.
Belon C, Rosas Olvera M, Vives E, Kremer L, Gannoun-Zaki L, Blanc-Potard AB.
Future Microbiol. 2016;11(2):215-25. doi: 10.2217/fmb.15.134.
Insights into the smooth-to-rough transitioning in Mycobacterium bolletii unravels a functional Tyr residue conserved in all mycobacterial MmpL family members.
Bernut A, Viljoen A, Dupont C, Sapriel G, Blaise M, Bouchier C, Brosch R, de Chastellier C, Herrmann JL, Kremer L.
Mol Microbiol. 2016 Mar;99(5):866-83. doi: 10.1111/mmi.13283.
