Chazal Nathalie

Position

Professeur des Universités – Virology

Faculté de Médecine de Montpellier

Team

Players in the Pathogenesis of the Retroviral Infections

Contact

email : nathalie.chazal[at]irim.cnrs.fr

Phone : +33 (0)4 34 35 94 55

Nathalie Chazal is a member of the Faculty of Medicine (University of Montpellier). She teaches Virology. In addition, Nathalie Chazal co-founded the Erasmus Mundus Master EDAMUS and created the national training CNRS Entreprises “pathogens and BSL3 “. More recently, Nathalie Chazal has developed an International University Diploma (DUI: Emerging Infections: One Health approach take off #1 MUSE), an International Master (Infectious Diseases and One Health, take off #2 MUSE).

In parallel to her teaching activity, Pr. Nathalie Chazal carries out her research activity at IRIM in the team led by Dr. JM. Mesnard and Dr. B. Beaumelle. Her research work is part of the theme dedicated to the study of the role of the HIV-1 antisense protein ASP. She is particularly interested in the links between ASP and autophagy, ASP and viral replication as well as in the structural and evolutionary aspects of ASP. She is currently co-directing the thesis of Abla Houmey and supervising a student of the Master 2 Biology-Health, Lise Holsteyn.

In addition to her research and teaching activities, Pr. Nathalie Chazal has several administrative responsibilities: Representative of the Infectiology axis within the MUSE Biology-Health Cluster, member of the MicrobiOccitanie committee, member of the management board of the Faculty of Medicine, member of the Innovation and Scientific Coordination Committee of IRIM, member of the scientific committee “Research in Society” Maison des Sciences de l’Homme SUD, scientific referent for “Montpellier Advanced Knowledge Institute of Transitions, MAKIT”.

Publications:

1. Roger H Miller, Alexis Zimmer, Gilles Moutot, Jean-Michel Mesnard and Nathalie Chazal. Retroviral Antisense Transcripts and Genes: 33 Years after First Predicted, a Silent Revolution? Viruses (2021). Nov 4;13(11):2221. doi: 10.3390/v13112221.
   
2. Chazal, N. Coronavirus, the king who wanted more than a crown: SARS-CoV-2 origin, biology and accessory genes. Submitted.
3. Savoret. J., Mesnard. JM., Gross, A and Chazal. N. Antisense Transcripts and Antisense Protein: A New Perspective on Human Immunodeficiency Virus Type 1. (2021) Front Microbiol. 2021 Jan 12;11:625941. doi: 10.3389/fmicb.2020.625941.
4. Savoret J, Chazal N, Moles JP, Tuaillon E, Boufassa F, Meyer L, Lecuroux C, Lambotte O, Van De Perre P, Mesnard JM, Gross A. A pilot study of the humoral response against the AntiSense Protein (ASP) in HIV-1-infected patients. (2020). Front Microbiol. Jan 24;11:20. doi:10.3389/fmicb.2020.00020
5. Chazal N, de Rocquigny H, Roussel P, Bouaziz S, Barré-Sinoussi F, Delfraissy JF, Darlix JL. The three lives of Pierre Boulanger. (2020). Retrovirology. 2020 Apr 30;17(1):9. doi: 10.1186/s12977-020-00518-0.
6. Neyret A, Gay B, Cransac A, Briant L, Coric P, Turcaud S, Laugâa P, Bouaziz S, Chazal N. Insight into the mechanism of action of EP-39, a bevirimat derivative that inhibits HIV-1 maturation. (2019). Antiviral Res. 2019 Apr;164:162-175. doi: 10.1016/j.antiviral.2019.02.014
7. Is Uracil-DNA Glycosylase UNG2 a New Cellular Weapon Against HIV-1? Kara H, Chazal N, Bouaziz S. (2019) Curr HIV Res. 2019;17(3):148-160. doi: 10.2174/1570162X17666190821154331.
8. Matkovic R, Bernard E, Fontanel S, Eldin P, Chazal N, Hassan Hersi D, Merits A, Péloponèse JM Jr, Briant L. The host DHX9 DExH Box helicase is recruited to Chikungunya virus replication complexes for optimal genomic RNA translation. (2019). Journal of Virology. 2019 Feb 5;93(4):e01764-18. doi: 10.1128/JVI.01764-18
9. Majdoul, J. Cosette², AK. Seye, E. Bernard, S. Frin, N. Holic, N. Chazal, L. Briant, L. Espert, A.Galy and D. Fenard. Relief of lentiviral vector entry restriction using peptides derived from Beclin1 and Beclin2 evolutionary conserved domains. (2017). J Biol Chem. 2017 Nov 10;292(45):18672-18681. doi: 10.1074/jbc.M117.800813.
10. Chazal and L. Briant. Chikungunya Virus: Advances in Biology, Pathogenesis, and Treatment. Chikungunya Virus Entry and Replication (2016). Springer (Editors: Okeoma,Chioma M).
11. Fargette M, Frutos^, R, Merlin A, Ravel P, Baskoro Tunggul Satoto T, Andayani E, Damayanti S, Kister G, Nghia ND, Bardie Y, Cornillot E, Devaux C, Moulia C, Gavotte L, Briant L, Chazal N & Libourel. (2015). Observatoire Scientifique en Appui à la GEstion Sanitaire sur un territoire (OSAGE-S). Dynamiques Environnementales. https://doi.org/10.4000/dynenviron.962
12. Bernard, E., Hamel, R., Neyret, A., Ekchariyawa, P., Molles, jp., Simmons, G., Chazal, N., Desprès, P., Missé, D & Briant, L. Human Keratinocytes restric CHikungunya virus replication at post-fusion step. (2015). 2015 Feb;476:1-10. doi: 10.1016/j.virol.2014.11.013.
13. Besteiro, S., Blanc-Potard A, Bonazzi M, Briant L, Chazal N, Cornillot E, Lentini G, Matkovic, R. Sanosyan A, Tuaillon E, Van de Perre P. Montpellier Infectious Diseases – Pôle Rabelais (MID) 3rd annual meeting (2015). Infect Genet Evol. Jun;32:161-4. doi:10.1016/j.meegid.2015.03.007.
14. Bahraoui E, Briant L, Chazal N. E5564 inhibits immunosuppressive cytokine IL-10 induction promoted by HIV-1 Tat protein. (2014). J. 2014 Dec 4;11:214. doi: 10.1186/s12985-014-0214-z.
15. Eldin, P., Chazal, N., Fenard, D., Bernard, E Guichou, JF., Briant, L. Vpr expression abolishes the capacity of HIV-1 infected cells to repair uracilated DNA. (2014). Nucleic Acids Research. 2014 Feb;42(3):1698-710. doi: 10.1093/nar/gkt974.
16. Giroud,C., Chazal, N., Brun, S., Gay, B & Briant, L. HIV-1-associated PKA acts as a cofactor for genome reverse transcription. (2013). 2013 Dec 17;10:157. doi: 10.1186/1742-4690-10-157.
17. Patramool, S., Bernard^, , Hamel, R., Natthanej, L., Chazal, N., Surasombatpattana, P., Daoust, S., Thongrungkiat, S., Thomas, T Briant, L & Missé^, D. Isolation of infectious Dengue virus and Chikungunya virus using anionic polymer-coated magnetic beads. (2013). J. Virological Methods. 2013 Oct;193(1):55-61. doi: 10.1016/j.jviromet.2013.04.016.
18. Bastien P, Braun-Breton C, Chazal N, Devaux C, Foulongne V, Frank M, Hernandez JF,Kremer L, Lebrun M, Moing VL, Leonetti JP, Lionne C, Peyrottes S. Montpellier Infectious Diseases (MID) 2(nd) Annual Meeting. (2013). Infect Genet Evol. 2013 Jun;16:450-4. doi: 10.1016/j.meegid.2013.01.019.
19. Coric, P., Turcaud, S., Souquet, F., Briant, L., Gay, B., Royer, J., Chazal, N., & Bouaziz, B. Synthesis of new derivatives of Beverimat whith higher activity against HIV-1 maturation. (2013). European Journal of Medicinal Chemistry. 2013 Apr;62:453-65. doi: 10.1016/j.ejmech.2013.01.013.
20. Gay, B., Bernard, E., Solignat, M., Chazal, N., Devaux, C., Briant L. pH-dependent entry of chikungunya virus into Aedes albopictus cells. (2012). Infect Genet Evol. 2012 Aug;12(6):1275-81. doi: 10.1016/j.meegid.2012.02.003.
21. Devaux, C., Bernard, E., Gay, B., Chazal, N. & Briant, L. Insect cell endocytosis of chikungunya virus adapted to Aedes albopictus, a mosquito recently introduced into southern France. (2012). Retrovirology. 9 (Suppl 1).
22. Chazal, N^* & Giroud, C^*.& Briant, L. Cellular kinases incorporated into HIV-1 particles: passive or active passengers ? (2011). Retrovirology. * equal contribution 2011 Sep 2;8:71. doi: 10.1186/1742-4690-8-71.
23. Briant,L., Gay, B., Devaux, C & Chazal, N. HIV-1 assembly, release and maturation. (2011). World journal of AIDS. 1 No. 4, 2011, pp. 111-130. doi: 10.4236/wja.2011.14017.
24. Bernard, E., Solignat, M., Gay,B., Chazal, N., Devaux,C & Briant, L. Endocytosis of chikungunya virus into mammalian  cells : role of clathrin and early endosomal compartments. (2010). Plos One. 2010 Jul 8;5(7):e11479. doi: 10.1371/journal.pone.0011479.
25. Brun, S., Chaloin, L ., Gay, B., Bernard, E., Devaux, C., Lionne, C., Chazal, N, & Briant, L. Electrostatic repulsion between HIV-1 capsid proteins modulates hexamer plasticity and in vitro assembly. (2010). Proteins: Structure, Function, and Bioinformatics. 2010 Jul;78(9):2144-56. doi: 10.1002/prot.22729.
26. Rayne F, Debaisieux S, Yezid H, Lin YL, Mettling C, Konate K, Chazal N, Arold ST, Pugnière M, Sanchez F, Bonhoure A, Briant L, Loret E, Roy C, Beaumelle B Phosphatidylinositol- (4,5)-bisphosphate enables efficient secretion of HIV-1 Tat by infected T-cells. (2010). EMBO J. 2010 Apr 21;29(8):1348-62. doi: 10.1038/emboj.2010.32.
27. Fenard, D., Houzet, L., Tupin, A., Brun, S., Solignat,M., Mougel,M., Devaux,C., Chazal, N. & Briant, L. Uracil DNA Glycosylase 2 negatively regulates HIV-1 LTR transcription. (2009). Nucleic Acids Research. 2009 Oct;37(18):6008-18. doi: 10.1093/nar/gkp673.
28. Brun, S., Solignat, M., Gay, B., Bernard, E., Chaloin, L., Devaux, C., Chazal, N & Briant, L. VSV-G pseudotyping rescues HIV-1 CA mutations that impair core assembly or stability. (2008). 2008 Jul 7;5:57. doi: 10.1186/1742-4690-5-57.
29. Chazal, N^*., Lazert, C^*., Briant,L., Gerlier, D & Cortay, Jl. Refined study of the interaction between HIV-1 p6 late domain and ALIX. (2008). Retrovirology. * equal contributionMay 13;5:39. doi: 10.1186/1742-4690-5-39.
30. Contreras, X., Bennasser, Y., Chazal, N., Moreau, M., Leclerc, C and E. Bahraoui. Human immunodeficiency virus type 1 Tat protein induces an intracellular calcium increase in human monocytes that requires DHP receptors: involvement in TNF-alpha production. (2005). 2005 Feb 5;332(1):316-28. doi: 10.1016/j.virol.2004.11.032.
31. Chazal, N. Assemblage, bourgeonnement et maturation du HIV-1. (2005). Virologie. Virologie, Vol. 9, n° 3, mai-juin 2005.
32. Gerlier,D., Alais, S. & Chazal. Les radeaux membranaires : des plates‐formes de choix pour l’entrée, l’assemblage ou le bourgeonnement de virus. (2004). Virologie. 2004;8(3):199-214.
33. Chazal, N & D. Gerlier. Virus entry, assembly, budding, and membrane rafts. (2003). Microbiol Mol Biol Rev. 2003 Jun;67(2):226-37, table of contents. doi: 10.1128/mmbr.67.2.226-237.2003.
34. Contreras, X., Bennasser, Y., Chazal, N, and E. Bahraoui. HIV-1 Tat induces TNF-alpha production by human monocytes: involvement of calcium and PKC pathways. (2003). J Soc Biol. 2003;197(3):267-75.
35. Bahbouhi, B., Chazal, N., Seidah, NG., Chiva, C., Kogan, M., Albericio, F., Giralt, E., & Bahraoui, E. Effects of L- and D-REKR amino acid-containing peptides on HIV and SIV envelope glycoprotein precursor maturation and HIV and SIV replication. (2002). Biochemical J. 2002 Sep 15;366(Pt 3):863-72. doi: 10.1042/BJ20020052.
36. Bardy, M., Gay, B., Pebernard, S., Chazal N., Courcoul, M., Vigne, R., Decroly, E., & Boulanger P. Interaction of human immunodeficiency virus type 1 Vif with Gag and Gag-Pol precursors: co-encapsidation and interference with viral protease-mediated Gag processing. (2001). Journal of General Virology. 2001 Nov;82(Pt 11):2719-2733. doi: 10.1099/0022-1317-82-11-2719.
37. Chazal, N., G. Singer., Aiken, C., Hammarskjöld, ML & Rekosh, D. Human immunodeficiency virus type 1 particles pseudotyped with envelope proteins that fuse at low pH no longer require Nef for optimal infectivity. (2001). Journal of Virology. 2001 Apr;75(8):4014-8. doi: 10.1128/JVI.75.8.4014-4018.2001.
38. Hong, SS., Galaup, A., Peytavi, R., Chazal, N & P. Boulanger. Human i4munodeficiency virus type 1 particles pseudotyped with envelope proteins that fuse at low pH no longer require Nef for optimal infectivity. (1999). Human Gene Therapy. 1999 Nov 1;10(16):2577-86. doi: 10.1089/10430349950016627.
39. Gay, B., Tournier, J., Chazal, N., Carrière, C. & Boulanger, P. Morphopoietic determinants of HIV-1 Gag particles assembled in baculovirus-infected cells. (1998). 1998 Aug 1;247(2):160-9. doi: 10.1006/viro.1998.9237.
40. Bouyac, M., M. Courcoul, G. Bertoia, Y. Baudat, D. Gabuzda, D. Blanc, Chazal, P. Boulanger., Sire, J., Vigne, R. & Spire, B. Human immunodeficiency virus type 1 Vif protein binds to the Pr55Gag precursor. (1997). Journal of Virology. 1997 Dec;71(12):9358-65. doi: 10.1128/JVI.71.12.9358-9365.1997.
41. Srinivasakumar, N., Chazal, N., Helga-Maria, C., Prasad, S., Hammarskjöld, ML. & Rekosh, D. et al., The effect of viral regulatory protein expression on gene delivery by human immunodeficiency virus type 1 vectors produced in stable packaging cell lines. (1997). Journal of Virology. 1997 Aug;71(8):5841-8. doi: 10.1128/JVI.71.8.5841-5848.1997.
42. Carrière, C., Gay,B., Chazal, N. & Boulanger, P. Sequence requirements for encapsidation of deletion mutants and chimeras of human immunodeficiency virus type 1 Gag precursor into retrovirus-like particles. (1995). Journal of Virology. 1995 Apr;69(4):2366-77. doi: 10.1128/JVI.69.4.2366-2377.1995.
43. Chazal, N., Gay, C. Carrière, and P. Boulanger. Human immunodeficiency virus type 1 MA deletion mutants expressed in baculovirus-infected cells: cis and trans effects on the Gag precursor assembly pathway. (1995). Journal of Virology. 1995 Jan;69(1):365-75. doi: 10.1128/JVI.69.1.365-375.1995.
44. Chazal, N., C. Carrière, B. Gay., and P. Boulanger. Phenotypic characterization of insertion mutants of the human immunodeficiency virus type 1 Gag precursor expressed in recombinant baculovirus-infected cells. (1994). Journal of Virology. 1994 Jan;68(1):111-22. doi: 10.1128/JVI.68.1.111-122.1994.

 

Brevet

Bouaziz S., Coric P., Boulanger P., Hong S. S., Turcaud S., Souquet F., Chazal, N., Briant L. “Nouveaux inhibiteurs d’assemblage et de maturation du VIH dérivés du Bevirimat (DSB, PA-457)” Brevet EP11306138.6. Date de dépôt 12/09/2011. Extension aux USA 2012.