: 04 July 2017

Viruses have a limited number of genes and need to divert the metabolism of their hosts for their own replication. Identification of cellular factors allowing the host to control the viral replication cycle is therefore a major issue to fight these pathogens. Our lab is interested in RNA viruses responsible for worldwide epidemics including AIDS viruses and emerging Chikingunya and Zika viruses. We are using genetic screens as well as imaging and molecular virology technics in order to identify genes involved in the susceptibility or resistance of viral infections and to decipher the mechanisms governing these processes. These approaches will also allow us to define the physiologic functions of these factors and to evaluate the impact of their dysfunction induced by these viruses.

Our projects are :

1- Identification of cofactors involved in Chikungunya and Zika arbovirus replication

* Structure et biogenesis of replication complexes at the host plama membrane .

* Role of cellular RNA helicases in RNA arbovirus transcription translation.

2 - Understanding the antiviral mecanisms targetting retroviral capsids

* Deciphering TRIM5alpha restiction using genetic screens.

* Identification of a novel antiviral protein in primate SIV transmission to humans.

3 - Study of viral interference to cellular metabolisme

* Epitranscriptomic et epigenetic modifications during neuroinfection ; consequences on viral replication and antiviral responses.

* Role the XPR1 retroviral receptor in phosphate homeostasis and calcification. Association of XPR1 to primary Familial Brain Calcification

Key words :

Host-pathogen interaction, viral replication virale, restriction, arbovirus, lentivirus, genetic screens, phosphate metabolism, calcification, neuroinfection.

2022

Tury S, Giovannini D, Ivanova S, Touhami J, Courgnaud V, Battini JL. Identification of Copper Transporter 1 as a Receptor for Feline Endogenous Retrovirus ERV-DC14. J Virol. doi: 10.1128/jvi.00229-22. Pubmed
Neyret A, Bernard E, Aïqui-Reboul-Paviet O, Bakhache W, Eldin P, Chaloin L, Briant L. Identification of a non-canonical G3BP-binding sequence in a Mayaro virus nsP3 hypervariable domain. Front Cell Infect Microbiol. doi: 10.3389/fcimb.2022.958176. Pubmed

2021

Kril V, Aïqui-Reboul-Paviet O, Briant L, Amara A. New insights into Chikungunya Virus Infection and Pathogenesis. Annu Rev Virol. doi: 10.1146/annurev-virology-091919-102021. Pubmed

2020

Bakhache W, Neyret A, Bernard E, Merits A, Briant L. Palmitoylated cysteines in Chikungunya virus nsP1 are critical for targeting to cholesterol-rich plasma membrane microdomains with functional consequences for viral genome replication. Journal of Virology. doi: 10.1128/JVI.02183-19. PubMed
Cassinari K, Rovelet-Lecrux A, Tury S, Quenez O, Richard AC, Charbonnier C, Olaso R, Boland A, Deleuze JF, Besancenot JF, Delpont B, Pouliquen D, Lecoquierre F, Chambon P, Thauvin-Robinet C, Campion D, Frebourg T, Battini JL, Nicolas G. Haploinsufficiency of the Primary Familial Brain Calcification Gene SLC20A2 Mediated by Disruption of a Regulatory Element. Mov Disord. doi: 10.1002/mds.28090. Pubmed
Clé M, Eldin P, Briant L, Lannuzel A, Simonin Y, Van de Perre P, Cabié A, Salinas S. Neurocognitive impacts of arbovirus infections. J Neuroinflammation. doi: 10.1186/s12974-020-01904-3. Pubmed
Eldin P, Péron S, Galashevskaya A, Denis-Lagache N, Cogné M, Slupphaug G, Briant L. Impact of HIV-1 Vpr manipulation of the DNA repair enzyme UNG2 on B lymphocyte class switch recombination. J Transl Med. doi: 10.1186/s12967-020-02478-7. Pubmed

2019

Bakhache W, Neyret A, McKellar J, Clop C, Bernard E, Weger-Lucarelli J, Briant L. Fatty acid synthase and stearoyl-CoA desaturase-1 are conserved druggable cofactors of Old World Alphavirus genome replication. Antiviral Research. 172:104642. PubMed
López-Sánchez U, Nicolas G, Richard AC, Maltête D, Charif M, Ayrignac X, Goizet C, Touhami J, Labesse G, Battini JL, Sitbon M. Characterization of XPR1/SLC53A1 variants located outside of the SPX domain in patients with primary familial brain calcification. Scientific Reports. 9(1):6776. PubMed
Bakhache W, Couderc É, Neyret A, Briant L. Architecture and biogenesis of positive-stranded RNA virus replication organelles. Virologie 23(3):160-175. PubMed
Neyret A, Gay B, Cransac A, Briant L, Coric P, Turcaud S, Laugâa P, Bouaziz S, Chazal N. Insight into the mechanism of action of EP-39, a bevirimat derivative that inhibits HIV-1 maturation. Antiviral Research. 164:162-175. PubMed
Matkovic R, Bernard E, Fontanel S, Eldin P, Chazal N, Hassan Hersi D, Merits A, Péloponèse JM Jr, Briant L. The Host DHX9 DExH-Box Helicase Is Recruited to Chikungunya Virus Replication Complexes for Optimal Genomic RNA Translation. Journal of Virology. 2019 Feb 5;93(4). PubMed

2017

Majdoul S, Cosette J, Seye AK, Bernard E, Frin S, Holic N, Chazal N, Briant L, Espert L, Galy A, Fenard D. Peptides derived from evolutionarily conserved domains in Beclin1 and Beclin2 enhance the entry of lentiviral vectors into human cells. J Biol Chem. 2017 Sep 19. PubMed
Mamede JI, Damond F, Bernardo A, Matheron S, Descamps D, Battini JL, Sitbon M, Courgnaud V. 2017. Cyclophilins and nucleoporins are required for infection mediated by capsids from circulating HIV-2 primary isolates. Sci Rep. 7:45214. PubMed
Wichit S., Hamel R., Bernard E., Talignani L., Diop F., Ferraris P., Liegeois F., Ekchariyawat P., Luplertlop N., Surasombatpattana P., Thomas F., Merits A., Choumet V., Roques P., Yssel H., Briant L. and Missé D. Imipramine Inhibits Chikungunya Virus Replication in Human Skin Fibroblasts through Interference with Intracellular Cholesterol Trafficking. Scientific Reports 7(1):3145 (2017). PubMed
Pham Thi KL, Briant L, Gavotte L, Labbe P, Perriat-Sanguinet M, Cornillot E, Vu TD, Nguyen TY, Tran VP, Nguyen VS, Devaux C, Afelt A, Tran CC, Phan TN, Tran ND, Frutos R. Incidence of dengue and chikungunya viruses in mosquitoes and human patients in border provinces of Vietnam. Parasites and Vectors. 2017 Nov 9;10(1):556. PubMed

2016

Anheim M, López-Sánchez U, Giovannini D, Richard AC, Touhami J, N'Guyen L, Rudolf G, Thibault-Stoll A, Frebourg T, Hannequin D, Campion D, Battini JL, Sitbon M, Nicolas G. 2016. XPR1 mutations are a rare cause of primary familial brain calcification. J Neurol. 263(8):1559-64. PubMed
Simonin Y, Loustalot F, Desmetz C., Foulongne V, Constant O., Fournier-Wirth C, LEON F., Molès JP, GOUBAUD A., LEMAITRE J.M., MAQUART M., LEPARC-GOFFART I., Briant L, Nagot N, Van de Perre P and Salinas S,. Zika Virus Strains Potentially Display Different Infectious Profiles in Human Neural Cells. EBioMedicine Oct;12:161-169 (2016). PubMed
Salinas S, Foulongne V, Loustalot F, Fournier-Wirth C, Molès JP, Briant L, Nagot N, Van de Perre P, Simonin Y. Zika virus an emerging threat. Med Sci (Paris). 2016 32(4):378-86. PubMed
Thi Pham LIEN K.; Briant L.; Tang T.B.; Trang B.M.; Gavotte L.; Cornillot E.; Duoc V.T.; Duong T.N., Frutos R; Nga P.T. Surveillance of dengue and chikungunya infection in Dong Thap, Vietnam: A 13-month study. Asian Pacific Journal of Tropical Medicine 9(1):39-43 (2016). PubMed

2015

Legati A., Giovannini D., Geschwind DH., Battini JL.*, Coppola G*. 2015. Mutations in XPR1 cause primary familial brain calcification associated with altered phosphate export. Nature Genetics 47: 579–581. PubMed
Bernard E, Hamel R., Neyret A., Ekchariyawat P., Molès J.P., Simmons G., Chazal N., Desprès P., Missé D. and Briant L. Human keratinocytes restrict Chikungunya virus replication at a post-fusion step. Virology. 476:1-10 (2015). PubMed
Nicolas G, Battini JL, Giovannini D, Sitbon M, Hannequin D. 2015. From the identification of the molecular bases of primary brain calcification to the disease mechanisms: New steps. Rev Neurol (Paris). 2015 Oct;171(10):685-7 PubMed
Peric D, Barragan I, Giraud-Triboult K, Egesipe AL, Meyniel-Schicklin L, Cousin C, Lotteau V, Petit V, Touhami J, Battini JL, Sitbon M, Pinset C, Ingelman-Sundberg M, Laustriat D, Peschanski M. 2015. Cytostatic Effect of Repeated Exposure to Simvastatin: A Mechanism for Chronic Myotoxicity Revealed by the Use of Mesodermal Progenitors Derived from Human Pluripotent Stem Cells. Stem Cells. 33(10):2936-48. PubMed
Ekchariyawat P., Hamel R., Bernard E., Wichit S., Surasombatpattana P. Talignani L., Thomas F., Choumet V., Yssel H., Desprès P., Briant L. and Missé D. Inflammasome signaling pathways exert antiviral effect against Chikungunya virus in human dermal fibroblasts. Infect. Genet. Evol. 32:401-408 (2015). PubMed
Sébastien Besteiro a, Anne Blanc-Potard a, Matteo Bonazzi b, Laurence Briant b, Nathalie Chazal b *, Emmanuel Cornillot b, Gaëlle Lentini a, Roy Matkovic b, Armen Sanosyan c, Edouard Tuaillon c, Philippe Van de Perre c. Montpellier Infectious Diseases - Pôle Rabelais (MID) 3rd annual meeting (2015). Infect. Genet Evol. 32:161-164 (2015).
Thi Kim Lien K., Duoc V.V., Gavotte L., Cornillot E., Nga P.T., Briant L., Frutos R., and Duong TN. Role of Aedes aegypti and Aedes albopictus during the 2011 dengue fever outbreaks in Hanoi, Vietnam. Asian Pacific Journal of Tropical Medicine 8:543-8 (2015). PubMed
Hamel R., Dejarnac O., Wichit S., Ekchariyawat P., Neyret A., Natthanej L., Perera-Lecoin M., Talignani L., Thomas F., Cao-Lormeau V., Choumet V., Briant L., Desprès P., Amara A., Yssel H. and Missé M. Biology of Zika virus infection in human skin cells. J. Virol. 1;89(17):8880-96 (2015). PubMed

2014

Eldin P., Chazal N., Fenard D., Bernard E., Guichou J.F., Briant L. Vpr expression abolishes the capacity of HIV-1 infected cells to repair uracilated DNA. Nucleic Acids Research. 1;42(3):1698-710.(2014). PubMed
Oburoglu L., Tardito S., Fritz V., De Barros S.C., Merida P., Craveiro M., Mamede J., Cretenet G., Mongellaz C., An X., Klysz D., Touhami J., Battini JL., Dardalhon V., Zimmermann V.S., Mohandas N., Gottlieb E., Sitbon M., Kinet S., Taylor N. 2014. Glucose and glutamine metabolism regulate human hematopoietic stem cell lineage specification. Cell Stem Cell 15: 169-184. PubMed
Joly J.P, Mata G., Eldin P., Briant L., Duca M. and Benhida R. Artificial nucleobase-amino acids conjugates: a new class of TAR RNA binding agents. Chemistry: a European Journal. 19, 1 – 10 (2014). PubMed
Briant L. Desprès P., Choumet V. and Missé D. Role of skin immune cells on the host susceptibility to mosquito-borne viruses. Virology. Sep; 464-465C:26-32 (2014). PubMed
Bahraoui E., Briant L. and Chazal N. E5564 inhibits immunosuppressive cytokine IL-10 induction promoted by HIV-1 Tat protein. Virology J. Dec 4;11(1):214 (2014). PubMed

2013

Giovannini D., Touhami J., Charnet P., Sitbon M., Battini JL. 2013. Inorganic phosphate export by the retrovirus receptor XPR1 in metazoans. 2013. Cell Reports 3: 1866-73. PubMed
Giroud C., Chazal N., Gay B., Eldin P., Brun S. and Briant L. HIV-1-associated PKA acts as a cofactor for genome reverse transcription. Retrovirology. 17;10(1):157 (2013). PubMed
Mamede JI., Sitbon M., Battini JL., Courgnaud V. 2013. Heterogeneous susceptibility of circulating SIV isolate capsids to HIV-interacting factors. Retrovirology 10: 77. PubMed
Laval J, Touhami J, Herzenberg LA, Conrad C, Taylor N, Battini JL, Sitbon M, Tirouvanziam R. 2013. Metabolic adaptation of neutrophils in cystic fibrosis airways involves distinct shifts in nutrient transporter expression. J Immunol.190(12):6043-50. PubMed
Patramool S., Bernard E., Hamel R., Natthanej. L, Chazal N., Surasombatpattana P., Ekchariyawat P., Daoust S., Thongrungkiat S., Thomas F., Briant L. and Missé D. Isolation of infectious Chikungunya virus and Dengue virus using anionic polymer-coated magnetic beads. Journal of Virological Methods. 193(1): 55-61 (2013). PubMed
Petit V, Massonnet G, Maciorowski Z, Touhami J, Thuleau A, Némati F, Laval J, Château-Joubert S, Servely JL, Vallerand D, Fontaine JJ, Taylor N, Battini JL, Sitbon M, Decaudin D. 2013. Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters. Lab Invest. 93(5):611-21. PubMed

2012

Loisel-Meyer S, Swainson L, Craveiro M, Oburoglu L, Mongellaz C, Costa C, Martinez M, Cosset FL, Battini JL, Herzenberg LA, Herzenberg LA, Atkuri KR, Sitbon M, Kinet S, Verhoeyen E, Taylor N. 2012. Glut1-mediated glucose transport regulates HIV infection. Proc Natl Acad Sci U S A. 109(7):2549-54. PubMed
Vaughan AE, Mendoza R, Aranda R, Battini JL, Miller AD. 2012. Xpr1 is an atypical G-protein-coupled receptor that mediates xenotropic and polytropic murine retrovirus neurotoxicity. J Virol. 86(3):1661-9. PubMed
B. Gay, E. Bernard, M. Solignat, N. Chazal, C. Devaux and L. Briant. pH-dependent entry of chikungunya virus into Aedes albopictus cells. Infection Genetics and Evolution. 12(6):1275-81 (2012). PubMed
Coric P., Turcaud S., Souquet F., Briant L., Gay B., ROYER J., Chazal N. and Bouaziz S. Synthesis and biological evaluation of new derivatives of bevirimat with higher activity against HIV-1 maturation. European Journal or Medicinal Chemistry. 62:453-65 (2013). PubMed